庚醇预处理的心脏保护作用对细胞膜缝隙连接蛋白43的影响  

Protective effect of heptanol preconditioning on heart and connexin 43

作　　者：何燕[1] 张正楠[1] 钟国强[1] 吴龙飞[1] 涂荣会[1] 文伟明[1] 陈卓宏[1] 李薇[1] 

机构地区：[1]广西医科大学第一附属医院老年心内科,南宁530021

出　　处：《中华老年心脑血管病杂志》2013年第4期407-411,共5页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：广西壮族自治区卫生厅科研课题(Z2011321)

摘　　要：目的探讨庚醇预处理对家兔心肌缺血再灌注损伤的作用及其机制。方法将50只新西兰大白兔随机分为5组:假手术组、缺血再灌注组(IR组)、缺血预处理组(IP组)、庚醇预处理组(HP组)、庚醇预处理加5-羟葵酸(5-HD)预处理组(HP+5-HD组),每组10只。所有新西兰大白兔再灌注4h后处死。分别于术前、缺血时、再灌注2、4h检测血浆肌酸激酶同工酶和心肌肌钙蛋白活性;采用免疫荧光标记检测Cx43。结果与IR组比较,IP组及HP组心肌坏死区/左心室范围明显降低(P<0.01)。与假手术组比较,IR组、HP+5-HD组Cx43mRNA表达明显降低(P<0.01);与HP+5-HD组比较,IP组、HP组Cx43mRNA表达明显升高(P<0.01);与IR组比较,IP组、HP组、HP+5-HD组Cx43mRNA表达明显升高(P<0.05,P<0.01)。结论庚醇预处理可以通过衰减由心肌缺血再灌注诱导的细胞膜Cx43表达下降,对损伤心肌起到保护作用。Objective To study the effect of heptanol preconditioning on myocardial ischemia/ reperfusion（I/R） injury in rabbits and its mechanism. Methods Fifty New Zealand white rabbits were randomly divided into sham operation group, I/R group, ischemia preconditioning （IP） group, heptanol preconditioning （HP） group and HP＋ 5-HD group （10 in each group）. The rabbits were killed 4 h after reperfusion. Their plasma CK-MB and cTnI activities were measured before operation,at ischemia, 2 and 4 h following reperfusion, respectively. Connexin43 （Cx43） expres- sion was detected by immunofluorescence labeling. Results The myocardial infarction size was significantly smaller in IP and HP groups than in IR group（P（0.01）. The Cx43 mRNA expression level was significantly lower in I/R and HP＋5-HD groups than in sham operation group （P〈0. 01） ,significantly higher in IP and HP groups than in HP＋5-HD group（P〈0.01） and in IP,HP and HP＋5-HD groups than in I/R group（P〈0.05,P〈0.01）. Conclusion Heptanol pre- conditioning can protect myocardium against injury by down-regulating myocardial I/R-induced Cx43 expression.

关 键 词：庚醇 心肌再灌注损伤 缺血预处理 连接蛋白43 细胞膜 

分 类 号：R965[医药卫生—药理学]