Aβ对大鼠海马细胞钙浓度和线粒体膜电位影响  

Effect of β-amyloid on intracellular calcium concentration and mitochondrial membrane potential of primary cultured hippocampal neurons

作　　者：吴艳萍[1] 马若波[1] 郑晶[2] 任锐[1] 

机构地区：[1]哈尔滨医科大学公共卫生学院卫生毒理学教研室,黑龙江哈尔滨150081 [2]黑龙江省疾病控制中心

出　　处：《中国公共卫生》2013年第4期521-523,共3页Chinese Journal of Public Health

基　　金：国家自然科学基金(30972503)

摘　　要：目的探讨β-淀粉样蛋白(Aβ25～35)对原代培养大鼠脑海马细胞内游离钙离子浓度和线粒体膜电位影响。方法原代培养8 d的SD大鼠脑海马神经细胞,利用老化的Aβ25～351、10和20μmol/L对其染毒,分别观察染毒后24、48和72 h时海马细胞形态改变、细胞内游离钙离子浓度和线粒体膜电位。结果随着染毒剂量增加,海马神经细胞开始变形、萎缩、神经元胞体模糊,突起变细、变短、分支减少,边缘模糊,神经网络破裂中断;电镜结果显示,随着染毒剂量增加和染毒时间延长,海马细胞凋亡比例增加;20μmol/L Aβ25～35染毒24、48和72 h后,细胞内Ca2+浓度分别为(30.79±1.28)、(38.19±2.13)和(41.65±3.60),细胞内线粒体膜电位分别为(46.94±9.55)、(39.98±6.51)和(34.52±5.67),与对照组比较,Ca2+浓度均升高,膜电位均降低(P<0.01)。结论 Aβ25～35可以通过破坏细胞内钙稳态和线粒体膜电位而发挥神经毒性作用。Objective To assess the effect of the β-amyloid fragment Aβ25-35 on mitochondria membrane potential and the concentration of intracellular calcium ion in primary hippocampal neurons. Methods Primary hippocampal neu- rons on the 8th day in vitro were treated with Aβ25-35 at the concentration of 1 μmol/L,10 μmol/L,and 20 μmol/L for 24 hr,48 hr, and 72 hr with double distilled water as control. Purity of primary hippocampal neurons was determined on the eighth day after plating. The morphology of primary hippocampal neurons was determined by light microscope and electron microscopical. [Ca^2＋] i and mitochondrial membrane potential were also determined. Results The results showed that Aβ25-35 damaged the morphology and mitochondria function of primary hippocampal neurons. For 20 ixmol/L Aβ25-35 treatment group,the concentration of Ca^2＋ at 24,48,and 72 hr was 30.79 ± 1.28,38. 19±2.13,and 41.65±3.60, significantly higher than that of the control group＇ and the mitochondrial membrane potential was 46.94 ± 9.55,39. 98±6.51 ,and 34.52±5.67 ,respectively, significantly lower than that of the control group. The results indicated that Aβ25-35 decreased mitochondrial membrane potential and increased[Ca^2＋] i in a dose-time dependent manner. Conclusion Aβ25-35 exhibits a neurotoxicity effect by interfering the calcium homeostasis and disturbing the function of mitochondrial.

关 键 词：β-淀粉样蛋白(Aβ25~35) 线粒体 游离钙离子 膜电位 

分 类 号：R33-33[医药卫生—人体生理学]