NFATc1在氟中毒大鼠破骨细胞中表达意义  

Expression of NFAT mRNA and protein in osteoclasts of rats with chronic fluorosis

作　　者：谢莹[1] 于燕妮[1] 陈锡山[1] 万良斌[1] 

机构地区：[1]贵阳医学院病理学教研室,贵州贵阳550004

出　　处：《中国公共卫生》2013年第4期530-533,共4页Chinese Journal of Public Health

基　　金：科技部国际合作项目(2010DFB30530);贵州省科技厅社会发展攻关项目(黔科合SY[2011]3006号)

摘　　要：目的探讨活化T细胞核因子(NFAT)在慢性氟中毒大鼠氟骨症破骨细胞中的作用。方法将36只SD大鼠按体重随机分为3组(每组12只,雌雄各半):对照组(饮水含氟<0.5 mg/L)、低氟组(5.0 mg/L)、高氟组(50.0 mg/L),实验8个月后股动脉放血处死大鼠,取大鼠股骨下端,抗酒石酸酸性磷酸酶染色(TRAP)法进行破骨细胞分化鉴定;免疫组织化学法和原位杂交检测各组大鼠股骨组织中NFATc1蛋白及其mRNA表达。结果NFATc1在破骨细胞中表达阳性,与对照组[(135.90±1.03),(110.45±1.55)]比较,低氟组大鼠破骨细胞中NFATc1蛋白及mRNA[(156.81±1.26),(132.50±1.58)]表达均升高(P<0.05),高氟组大鼠破骨细胞中NFATc1蛋白及mRNA[(135.46±1.19),(110.26±1.37)]均呈下降趋势,但差异无统计学意义(P>0.05)。结论NFATc1可能是氟中毒氟骨症破骨细胞分化调节的重要环节。Objective To study the role of nuclear factor of activated T cell 1 （NFATcl） in the osteoclasts of rats with skeletal fluorosis caused by chronic fluorosis. Methods Thirty-six Sprague-Dawley rats were randomly divided into three groups according to their body weight （ 12 in each group, half male and half female） ：control group （ sodium fluoride [NaF] 〈0.5 rag/L） ,low-dose fluoride group（5.0 mg/L NaF） ,high-dose fluorosis group（50. 0 mg/L NaF）. The rats were sacrificed after 8 months of fluoride treatment. The number of osteoclast inside the distal femur of the rats was coun- ted by tartrate-resistant acid phosphatase staining（TRAP） ,and the protein and mRNA levels of NFATcl inside the distal femur of the rats were detected by immunohistochemistry and in-situ hybridization. Results NFATcl positive osteoclasts were observed. Compared to the control group（ 135.90±1.03,110.45±1.55 ） ,the protein and mRNA expressions of NFATcl were higher （ 156.81±1.26,132.50±1.58） in the low-dose fluoride group （135.46±1.19,110.26±1.37） （P 〈0.05） , but decresed in the high-dose fluoride groups （P〉0.05）. Conclusion NFATcl may play an important role in osteoclasts differentiation and regulation in the skeletal fluorosis caused by chronic fluorosis.

关 键 词：氟骨症 破骨细胞 活化T细胞核因子(NFAU) 

分 类 号：R599[医药卫生—内科学]