微囊藻毒素-LR对小鼠肝甲基转移酶活性影响  

Effect of microcystin-LR on DNA methyltransferase activity in mouse liver

作　　者：何灏逾[1] 农清清[1] 韦宏旷[1] 陆继培[1] 李春宏[1] 荆敏[2] 

机构地区：[1]广西医科大学公共卫生学院职业卫生与环境卫生学教研室,广西南宁530021 [2]广西医科大学第一附属医院检验科

出　　处：《中国公共卫生》2013年第4期533-535,共3页Chinese Journal of Public Health

基　　金：广西自然科学基金(2012GXNSFAA053155);广西高等学校重点资助科研项目(201202ZD024);2011年广西高校优秀人才资助计划项目

摘　　要：目的观察微囊藻毒素-LR(MCLR)暴露对小鼠肝组织DNA甲基转移酶(DNMTs)活性的影响。方法 80只昆明小鼠,雌雄各半,随机分为4组,每组20只,即对照组(生理盐水)及MCLR低、中、高剂量组(MCLR5、10、20μg/kg),1次/d经腹腔注射染毒,分别于染毒第11、21 d测定肝功能生化指标,观察肝脏组织形态学变化,检测DNMTs酶活性。结果染毒11 d时,与对照组比较,低剂量MCLR组小鼠肝脏谷丙转氨酶(ALT)、谷草转氨酶(AST)和碱性磷酸酶(ALP)无明显变化,高剂量组ALT、AST、ALP酶活性均升高,差异有统计学意义(P<0.05);染毒21 d时,与对照组比较,各剂量MCLR组小鼠肝脏ALT和AST水平均升高,差异有统计学意义(P<0.05);染毒11、21 d时,低、中、高MCLR组小鼠DNMTs酶活性分别为(89 135.7±14 334.7)、(92 645.8±15 962.3)、(70481.3±15 621.3)和(59 115.6±9 884.6)、(83 310.7±15 066.3)、(86 612.5±20 572.8)RFU/(h.mg),均高于对照组,差异有统计学意义(P<0.05)。结论 MCLR暴露可引起ALT、AST、ALP水平升高,肝组织DNMTs酶活性升高。Objective To observe the effects of microcystin-LR （MCLR） on methyltransferase activity in mice liver. Methods Eighty mice were randomly divided into 0,5,11 and 21 μg/kg group according to the dose of MCLR. MCLR was administered for 10 and 20 days, respectively. The liver function and DNA methyltransferase activity were determined. Morphological changes of livers were observed. Results Compared with the 0 μg/kg group, the levels of alanine aminotransferase （ALT）, aspartate aminotransferase （ AST ）, alkaline phosphatase （ALP） in serum of the 5 μg/kg group were not obviously different after the exposure of 11 days. The levels of ALT, AST, and ALP in 20 μg/kg group were statistically significantly higher than that of 0 μg/kg group（ P〈0.05） after the exposure of 11 days. The levels of ALT and AST in 5,10,20 μg/kg group were statistically significantly higher than that of 0 μg/kg group（ P 〈 0.05） after the exposure of 21 days. The DNA methyltransferase activity in 5,10, and 20 μg/kg group exposed for 11 days were 89 135.7±14 334. 7,92 645.8±15 962. 3 ,and 70 481.3±15 621.25 relative fluorescence unit（ RFU）/h/mg,respectively, with statistical differences compared with that of 0 μg/kg group （ P〈0.05 ）. The DNA methyltransferase activity in 5,10,and 21 μg/kg group exposed for 20 days were 59 115.6±9 884. 6,83 310. 7±15 066. 3, and 86 612.5±20 572. 8 RFU/h/mg ,respectively,with statistical differences compared with that of 0 μg/kg group （ P 〈 0.05）. Conclusion MCLR could induce the increases of ALT,AST,ALP levels and up-regulation of methyltransferase activity in mice liver.

关 键 词：微囊藻毒素-LR(MCLR) DNA甲基转移酶(DNMTS)活性 肝功能 

分 类 号：R33[医药卫生—人体生理学]