HPLC法测定宁夏枸杞中法荜枝苷的含量  

Determination of Fabiatrin in Lycii Fructus by HPLC

作　　者：徐飞[1] 王晓中[2] 龚波林[2] 

机构地区：[1]宁夏回族自治区疾病预防控制中心,宁夏750004 [2]宁夏大学能源化工重点实验室,宁夏750021

出　　处：《天然产物研究与开发》2013年第3期363-366,共4页Natural Product Research and Development

基　　金：国家科技支撑计划子项目(2009BAI72B00);宁夏回族自治区科技攻关计划项目;宁夏大学科学研究基金(ZR1144)

摘　　要：本文采用高效液相色谱法,建立宁夏枸杞中法荜枝苷的含量测定方法。色谱条件:Diamonsil C8柱(150mm×4.6 mm,5μm);流动相为A:乙腈-水-醋酸(5∶94.5∶0.5),B:乙腈-水-醋酸(72∶27.5∶0.5),梯度洗脱。流速:1 mL/min;柱温:30℃;检测波长:346 nm。结果表明,在0.1～10μg/mL范围内线性关系良好,相关系数r=0.9998(n=8)。法荜枝苷的平均回收率为99.1%,RSD为1.55%。该方法准确可靠,可用于测定宁夏枸杞中法荜枝苷的含量,为宁夏枸杞开发利用提供理论依据。A high-performance liquid chromatography （HPLC） method was established for the detection and quantification of fabiatrin in Lyeii Fructus. Chromatographic separation was performed on Diamonsil Cs column （ 150 mm× 4.6 mm,5 μm）,using aeetonitrile-water-aeetie acid （5：94.5：0.5 ） as mobile phase A and aeetonitrile-water-aeetic acid （72： 27.5：0.5 ） as mobile phase B with gradient elution. The flow rate was 1 mL/min at 30 ℃ ,and the detection wavelength was set at 346 nm. The result showed that the linearity of fabiatrin in the range of 0.1 - 10 μg/mL （ r = 0.9998, n = 8 ） was good. The average recovery of fabiatrin was 99.1%, RSD was 1.55 %. The method was accurate, reliable, and can be used as a quality control method for fabiatrin in Lyeii Fructus. It provided the basis for development and utilization of Lyeii Fructus.

关 键 词：宁夏枸杞 法荜枝苷 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]