甜菜线粒体atp6基因RNA编辑位点研究  

The editing sites in the mitochondrial atp6 gene transcripts of sugar- beet Cytoplasmic Male Sterile line

作　　者：施真[1] 程大友[1,2] 

机构地区：[1]哈尔滨工业大学生命科学与技术学院,哈尔滨150001 [2]哈尔滨工业大学食品科学与工程学院,哈尔滨150001

出　　处：《中国甜菜糖业》2013年第1期12-17,共6页China Beet & Sugar

基　　金：国家自然科学基金（30771373）;黑龙江省自然科学基金（C200807）.

摘　　要：RNA编辑现象普遍存在于高等植物的线粒体中，是线粒体中产生功能蛋白所不可或缺的加工步骤。以甜菜细胞质雄性不育系DY5-CMS及其保持系DY5-O为材料，通过PCR、RT—PCR以及PCR产物直接测序等方法，对甜菜线粒体atp6基因转录本的RNA编辑位点进行了研究。结果表明，啤而基因转录本的保守区域共有8个编辑位点，均发生在密码子的第一、二位点上，这些位点的编辑将导致氨基酸种类的变化，如第二个编辑位点上发生的由丝氨酸（疏水参数0．8）到亮氨酸（疏水参数3．8）的转变。通过分析可看出atp6基因RNA编辑有着改变蛋白质疏水性的作用。The phenomenon of RNA editing exits extensively in mitochondrial genome of the higher plant, and is an essential process for producing functional proteins in mitochondria. Using the sugar - beet Cytoplasmic Male Sterility line DY5 -CMS and maintainer line DY5 -O as the research materials, we studied the editing sites in the mitochondrial atp6 gene transcripts of sugar - beet by PCR, RT - PCR and PCR products direct sequencing methods. The results showed that DY5 -O and DY5 -CMS share the same 8 editing sites found in conservative area of atp6 gene transcripts. All of the editing sites were C - U conversion and they all occurred at the 1 st or the 2nd position of codons, which might change the amino acid residues type, such as the transformation of Ser to Leu in the second editing site. Moreover, The RNA editing of mitochondrial atp6 gene of sugar - beet can alter protein＇ s hydrophobicity and increase the conservation among species.

关 键 词：甜菜 线粒体 RNA编辑 atp6基因 细胞质雄性不育 

分 类 号：S566.3[农业科学—作物学]