外源GA_3和PP_(333)对甜樱桃新梢生长及赤霉素代谢关键基因表达的影响  

Effects of GA_3 And PP_(333) on Shoot Growth and Gene Expression of Gibberellins Metabolism in Prunus Avium

作　　者：刘芳[1] 袁华招[1] 沈欣杰[1] 廖雄[1] 李天红[1] 

机构地区：[1]中国农业大学农学与生物技术学院/北京市果树逆境生理与分子生物学重点开放实验室,北京100193

出　　处：《核农学报》2013年第3期272-278,共7页Journal of Nuclear Agricultural Sciences

基　　金：公益性行业(农业)科研专项(20100321);国家自然科学基金(31171938)

摘　　要：本研究以9年生甜樱桃红灯(Prunus avium L.‘Hongdeng’)为试材,于新梢生长期进行100mg.L-1赤霉素(GA3)、300 mg.L-1多效唑(PP333)叶面喷施处理。试验结果表明GA3可明显促进新梢生长,新梢生长量长达15.04cm,为对照的2倍,而PP333则显著抑制了新梢的生长。GA3处理可使其内源GA3含量发生明显变化,在处理后14d更高达对照的2.2倍,之后迅速下降,而PP333处理后内源GA3含量呈持续下降趋势。实时荧光定量PCR结果表明GA3处理能够抑制GA20-ox、GA3-ox 2种合成关键酶基因的转录,而PP333处理则使GA20-ox、GA3-ox转录水平在后期明显升高。同时赤霉素代谢关键酶GA2-ox转录水平在不同处理中均表现出与赤霉素含量类似的变化规律。说明在甜樱桃内源赤霉素含量与合成代谢酶转录水平间,反馈调节机制可能发挥着重要作用。该研究结果将为生产中采用基因工程手段控制营养生长,实现矮化密植提供理论参考。At shoot-growing stage,100 mg·L-1 GA3 and 300 mg·L-1 PP333 were sprayed on the leaves of 9-year-old sweet cherry（Prunus avium L.＇Hongdeng＇）.Results showed that GA3 promoted the growth of shoots,and the average length increased by 15.04 cm,which was two-fold of control,and PP333 significantly inhibited the growth.The endogenous GA3 level increased with leaf-spraying GA3,which was 2.2 times of CK at 14 days after treatment and followed by a rapid decline.The endogenous GA3 level after PP333 treatment showed a continuing downward trend.Real-time PCR results showed that： GA3 inhibited the transcription of GA3-ox and GA20-ox（two gibberellins synthetic enzyme genes）.Transcription levels of GA3-ox and GA20-ox after PP333 treatment were significantly higher in the late stages.The variation of GA2-ox（a key enzyme in gibberellins metabolism） might play an important role between the transcription level of anabolic enzymes genes and endogenous gibberellins level.So,feedback regulation mechanism might play an important role between the transcription level of anabolic enzymes genes and endogenous gibberellins level.These results would provide a theoretical reference for using genetic engineering methods to control vegetative growth during production to achieve dwarf and high dense planting consequently.

关 键 词：甜樱桃 赤霉素 多效唑 新梢生长 基因表达 

分 类 号：S662.5[农业科学—果树学]