两个低植酸大豆突变体中肌醇-3-磷酸合成酶MIPS1基因表达特性的研究  

Characterization of D-myo-inositol 3-phosphate Synthase Gene Expression in Two Soybean Low Phytate Mutants

作　　者：袁凤杰[1] 董德坤[1] 李百权[1] 郁晓敏[1] 傅旭军[1] 朱丹华[1] 朱申龙[1] 杨清华[1] 

机构地区：[1]浙江省农业科学院作物与核技术利用研究所,浙江杭州310021

出　　处：《核农学报》2013年第3期286-292,共7页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金资助项目(30871542)

摘　　要：肌醇-3-磷酸合成酶(MIPS)基因是植酸合成代谢途径中重要的功能基因。本研究以两个大豆低植酸突变体Gm-lpa-TW-1、Gm-lpa-ZC-2以及其野生型亲本台湾75和浙春3号为材料,分析了MIPS1基因在其根、茎、叶、花和发育种子中转录水平上的表达特性。结果表明:在大豆发育的种子中MIPS1基因的表达表现为由弱到强再逐渐减弱的表达特性,在开花后22d的种子内,基因的表达达到高峰,此后逐渐减弱。突变体Gm-lpa-TW-1和野生型亲本台湾75的根、茎、叶和花中MIPS1基因的表达均较弱,但突变体Gm-lpa-TW-1的表达量略高于野生型亲本。与野生型亲本相比较,在开花后的22d,突变体Gm-lpa-TW-1发育种子中MIPS1基因的表达峰极显著的降低,仅为野生型的25%左右,而且在整个种子发育期间MIPS1基因的表达均较弱。突变体Gm-lpa-ZC-2和浙春3号的根、茎、叶和花中MIPS1基因的表达较弱,突变型和野生型之间没有显著的差异,但在种子发育的各个时期突变体Gm-lpa-ZC-2的表达量显著的高于野生型亲本浙春3号。在两个低植酸突变体发育的种子中MIPS1基因的表达与野生型亲本相比均发生了显著的变化,表明基因突变对MIPS1基因的表达均造成了显著的影响,在Gm-lpa-TW-1中表现为MIPS1基因表达的下调,而Gm-lpa-ZC-2中则表现为上调。1D-myo-inositol 3-phosphate synthase（MIPS） gene plays a significant role in phytic acid biosynthesis.In this study,we used two low phytic acid mutants Gm-lpa-TW-1,Gm-lpa-ZC-2 and their respective wild type parents Taiwan75 and Zhechun No.3 to analyze the expression pattern and characterization of MIPS1 gene.The results showed that there was a common expression pattern of MIPS1 in soybean developing seeds.Expression was weak as detected by RT-PCR in initial stage,increased in the following stages,and the peak expression was appeared in 22 day after flowering（DAF）.The expression of MIPS1 gene of non-seed tissues in mutant Gm-lpa-TW-1 and its wildtype Taiwan75 was very weak.In the developing seeds,the MIPS1 expression by qRT-PCR revealed a significant reduction in 22 DAF in mutant Gm-lpa-TW-1 as compared with the wildtype.Similarly,the expression of MIPS1 gene in non-seed tissue of Zhenchun No.3 and Gm-lpa-ZC-2 was very weak.However,stronger expression in developing seeds of the mutant Gm-lpa-ZC-2 than Zhechun No.3 was found.We concluded that the MIPS1 gene expression in the developing seed exhibited an up-regulation pattern in mutant Gm-lpa-ZC-2,but a down-regulation pattern in the mutant Gm-lpa-TW-1.

关 键 词：大豆 低植酸 MIPS1基因 表达特性 

分 类 号：S565.1[农业科学—作物学]