水稻LOC_Os05g02754基因的分子表征  

Molecular Characterization of LOC_Os05g02754 Gene in Rice(Oryza Sativa L.)

作　　者：王启钊[1,2,3] 赵海军[1,2,3] 李文旭[1,2,3] 刘国振[4] 舒庆尧[1,3] 

机构地区：[1]浙江大学原子核农业科学研究所/农业部浙江省核农学重点实验室,浙江杭州310029 [2]无锡-浙大生物农业中心,江苏无锡214100 [3]浙江大学水稻生物学国家重点实验室,浙江杭州310029 [4]河北农业大学生命科学学院,河北保定071001

出　　处：《核农学报》2013年第3期301-306,共6页Journal of Nuclear Agricultural Sciences

基　　金：农业部公益性行业科研专项(201103007);无锡市政产学研合作项目(CYES1002)

摘　　要：已有研究表明,LOC_Os05g02754基因很可能是控制水稻光叶性状的候选基因,光叶性状可能是该基因在第2外显子的5'端非翻译区的一个A→T的单核苷酸变异造成。本研究利用Western blot技术证实LOC_Os05g02754能够翻译成蛋白,但发现光叶和毛叶水稻品种间蛋白表达量不存在显著差异。通过构建该基因与黄色荧光蛋白基因的融合基因载体,并在水稻原生质体中的瞬时表达,将该蛋白定位在叶绿体中;同时,根据预测的二级结构、跨膜特性和信号肽等信息,推测该蛋白为一叶绿体跨膜蛋白。本研究首次试验验证了LOC_Os05g02754基因可以翻译成蛋白,并定位于叶绿体中,但研究结果不支持该基因控制水稻光叶特性。Previous studies suggested that the LOC_Os05g02754 gene is the candidate gene controlling trichome development and the A→T nucleotide mutation in the 5′ untranslated region of the 2nd exon of this gene might be responsible for the glabrous trait in rice.Western blot analysis demonstrated that LOC_Os05g02754 is translated into protein,however its expression levels in rice leaves were not significantly different between glabrous and pubescent rice cultivars.LOC_Os05g02754 was fused with yellow fluorescence protein gene and the heterologous gene was transiently expressed in rice protoplasts,the results demonstrated that LOC_Os05g02754 is localized in chloroplasts.Bioinformatics analysis of its predicted secondary structure,transmembrane helices and signal peptide also indicated that LOC_Os05g02754 is a protein spanning chloroplast membrane.The present study experimentally demonstrated that LOC_Os05g02754 gene is translated and its protein is localized in chloroplast.However,our results do not support the hypothesis that LOC_Os05g02754 gene controls the pubescent /glabrous trait in rice.

关 键 词：LOC_Os05g02754基因 光叶性状 亚细胞定位 叶绿体蛋白 水稻 

分 类 号：S511[农业科学—作物学]