干旱胁迫下燕麦叶片抗氧化酶活性等生理特性变化及抗旱性比较  

Comparative experiment on drought-resistant characteristics ofdifferent oat cultivars

作　　者：张娜[1] 赵宝平[1] 张艳丽[1] 任鹏[1] 刘景辉[1] 王莹[1] 

机构地区：[1]内蒙古农业大学燕麦科技创新团队,内蒙古呼和浩特010019

出　　处：《干旱地区农业研究》2013年第1期166-171,218,共7页Agricultural Research in the Arid Areas

基　　金：国家自然基金科学项目(31101126);内蒙古自然科学基金项目(2010DS0305);现代农业产业技术体系建设专项资金(CARS-08-B-5)

摘　　要：为选育抗旱型燕麦品种,以内蒙古主产区种植的21个燕麦品种为试验材料,采用盆栽方式在拔节期进行干旱处理(田间持水量25%,即土壤质量含水量为5%),测定叶片生理指标,综合比较其抗旱性,运用隶属函数法和聚类分析法进行分析,鉴定各品种的抗旱性。结果表明:坝莜8号、莜麦4400和燕2006的SOD活性分别升高了113.6%、150.36%和140.15%,坝莜8号的POD活性升高了99.1%;坝莜8号的脯氨酸含量升高了230.66%,而其可溶性蛋白含量仅下降9.6%,莜麦4400的丙二醛和相对电导率升高幅度较小,分别升高了13.4%和46.27%,其抗旱系数较大。隶属函数与聚类分析分类的相同程度为71.43%,综合分析21个品种的抗旱性可分为强抗旱型、抗旱型、中抗旱型、弱抗旱型4类。其中强抗旱型有两个品种,分别为坝莜8号和莜麦4400,抗旱型品种为燕2006和燕2004两个品种,中抗旱型的品种为白燕4号、青永久440和新源燕麦3个品种,其余品种为弱抗品种。For breeding the drought resistant oat varities, 21 oat cuhivars which were widely planted in Inner Mon- golia＇ s main producing area were used as materials in this experiment. At jointing stage, 25 % field capacity was used on oats for drought stress simulation, and physiological indexes in leaves relating to drought-resistant characteristics were an- alyzed in order to compare the drought resistance of each euhivar. The results showed that, the SOD activity of Bayou 8, Youmai 4400, Yan 2006 improved by 113.6 %, 150.36 % and 140.15 % respectively, while the POD activity of Bayou 8 improved by 99.1%. The praline of Bayou 8 improved by 230.66 %, while its protein decreased only by 9.6 %. The MDA and REC of Youmai 4400 improved by 14.3% and 46.27% respectively, while the index of its drought resistance was large. The synthetical drought resistance was estimated by membership function method and validated by clustering analysis, and the similarity degree of these two methods was 71.43%. The drought-resistant types of the 21 cultivars were divided into 4 types in the estimated result, and they were separately strong resistance, resistance, middle resis- tance and weak resistance. There were 2 cuhivars in the type of strong resistance, namely Bayou 8 and Youmai 4400. Drought-resistant cultivars were Yan 2004 and Yan 2006. Middle drought-resistant cultivars were Baiyan4, Qingyongjiu 4400 and Xinyuan. The other cuhivars were weak in drought resistance.

关 键 词：燕麦 抗旱性 生理指标 隶属函数 聚类分析 

分 类 号：S512.6[农业科学—作物学] S311