用于亲和分离的链霉亲和素磁性高分子微球的制备  

Preparation of Streptavidin Magentic Polymer Microsphere for the Affinity Separation

作　　者：梁媛媛[1] 温汉华[1] 郭卫强[1] 焦艳华[1] 

机构地区：[1]杭州师范大学生物医药与健康研究中心,浙江杭州311112

出　　处：《杭州师范大学学报（自然科学版）》2013年第2期145-149,共5页Journal of Hangzhou Normal University(Natural Science Edition)

基　　金：浙江省公益计划项目(2010C33132);杭州科技计划发展项目(20101133N04);杭州师范大学科研启动项目(PD10002004001040)

摘　　要：本文使用戊二醛作为偶联剂在氨基磁性高分子微球P(St-GMA-NH2)/Fe3O4的表面引入链霉亲合素(streptavidin,SA),制备得到链霉亲和素磁性高分子微球(P(St-GMA-SA)/Fe3O4).使用扫描电镜(SEM)、紫外光谱(UV)表征了P(St-GMA-SA)/Fe3O4的形貌与结构,探讨了反应时间,微球用量对微球表面SA引入量的影响,并考察了P(St-GMA-SA)/Fe3O4对生物素化羊抗人抗体(BIgG)的亲和吸附能力.结果表明当反应时间为12h,P(St-GMA-NH2)/Fe3O4用量为75mg时,SA的引入量可达到2.7μg/mg,P(St-GMA-SA)/Fe3O4在室温下能快速和BIgG结合,最大结合量为7.4μg/mg,P(St-GMA-SA)/Fe3O4与BIgG形成的复合物对热、酸、碱都表现出很好的稳定性.Streptavidin magnetic polymer microsphere P （St-GMA-SA）/Fe3O4 was prepared by introducing bimolecular streptavidin （SA） into the surface of P（St-GMA-NH2 ）/Fe3O4 with the aid of glutaraldehyde as crosslinking agent. The paper characterized the appearance and structure of P（St-GMA-SA）/Fe3O4 by SEM and UV, discussed the influences of reaction time and microsphere amount on the intake of SA, and investigated the affinity adsorption ability of P（St-GMA- SA）/Fe3O4 to BIgG. The results show that the intake of can reach to 2.7 μg/rag in 12 hours when the （St-GMA-NH2）/ Fe304 dosage is 75 mg. P（St-GMA-SA）/Fe3O4 is able to combine with BIgG to form stable complexes under mild conditions, which are stabile to heat, acid and alkali, and the maximum BIgG binding capacity is 7.4 μg/mg.

关 键 词：链霉亲和素 磁性高分子微球 亲和分离 

分 类 号：TB34[一般工业技术—材料科学与工程]