大鼠脾切除后自体脾细胞和脾片联合移植细胞免疫学研究  

Cellular immunity research on combination transplantation of antogeneic Splenic cell and slices after rat's splenectomy

作　　者：赵守魁[1] 郭利光[1] 范正军[2] 

机构地区：[1]安阳地区医院普外一科,455000 [2]郑州大学第一附属医院肝胆外科,450052

出　　处：《中国医疗前沿》2013年第5期29-30,81,共3页China Healthcare Innovation

摘　　要：目的通过建立脾细胞移植和脾片联合移植动物模型来观察脾片移植早期细胞免疫功能恢复状况。方法 Wistar大鼠48只,随机分为4组,A组为对照组,行脾切除术,B组为脾片移植组,C组为脾细胞移植组,D组为脾细胞脾片联合移植组,在建立模型前及模型成功后1周、4周、8周抽血,检测外周血T细胞亚群CD3+、CD4+、CD8+及CD4+/CD8+水平并进行比较。结果 A组脾切除后1、4、8周CD3+、CD4+、CD8+降低(P<0.05),B组移植后1、4周CD3+、CD4+、CD8+与术前相比降低(P<0.05),至8周逐渐接近正常(P>0.05),C组移植后1周CD3+、CD4+、CD8+与术前相比降低(P<0.05),至4周逐渐接近正常(P>0.05),而8周逐渐降低(P<0.05),D组术后1周CD3+、CD4+、CD8+与术前相比降低(P<0.05),4周和8周接近正常(P>0.05);CD3+、CD4+、CD8+在术后1、4周C和D组与A和B组有差异(P<0.05),在术后8周B和D组与A和C组有差异(P<0.05)。结论在脾片移植的基础上联合脾细胞移植能弥补脾片移植早期的部分细胞免疫功能。Objective To observe functional restoration of pristine cellular immunity after splenic sliices autotransplantation by establishing animal model on combination trantran-splantation of antogeneic Splenic cell and slices.Methods 48 wistar rats are randomly divid ed into four groups,A group： splenectomy.B group： splenic slices transplantation.C group： spleen cell transplantation.D group： combination transplantation.Before animal moddel and within 1,4,8 weeks after stablishing animal model,peripheral blood is drewn and detected the level of peripheral blood T-cyte subset CD3＋,CD4＋,CD8＋ and CD4＋/CD8＋,At the same time,the results are compared.Result In A group postoperative CD3＋,CD4＋,CD8＋ became lower（P 0.05）;In B group CD3＋,CD4＋,CD8＋ became lower compared with preoperative levels at the postoperative 1th and 4th week（P 0.05）,got close common at the postoperative 8th week（P 0.05）;In C group CD3＋,CD4＋,CD8＋ became lower compared with preoperative levels at the postoperative 1th week（P 0.05）,got close common at the postoperative 4th week（P 0.05）,but became gradually lower at the postoperative 8th week（P 0.05）;In D group CD3＋,CD4＋,CD8＋ became lower compared with preoperative levels at the postoperative 1thweek（P 0.05）,got close common at the postoperative 4th and 8th week（P 0.05）;Statistically difference was detected between the C,D and A,B groups for CD3＋,CD4＋,CD8＋ at the postoperative 1th and 4th week（P 0.05）,Statistically difference was detected between the B,D and A,C groups for CD3＋,CD4＋,CD8＋ at the postoperative 8th week（P 0.05）.Conclusion Combination trantransplantation of antogeneic Splenic cell and slices can retrieve partial pristinel cellular immune function.

关 键 词：脾切除 自体脾组织移植 脾脏 

分 类 号：R392.1[医药卫生—免疫学]