电纺聚己内酯-明胶纳米纤维膜复合兔骨髓间充质干细胞构建软骨组织工程支架  

Rabbit bone marrow mesenchymal stem cells seeding on electrospinning polycaprolactone/gelatin scaffold for cartilage repair

作　　者：徐正良[1] 刘骥[1] 张长青[1] 

机构地区：[1]上海交通大学附属第六人民医院骨科,200233

出　　处：《国际骨科学杂志》2013年第2期115-118,共4页International Journal of Orthopaedics

基　　金：国家自然科学基金青年科学基金项目(30801221)

摘　　要：目的探索构建电纺聚己内酯(PCL)-明胶纳米纤维膜复合体作为软骨组织工程支架的可行性。方法采用静电纺丝技术制作PCL-明胶(50︰50)纳米纤维膜作为支架。取第三代兔骨髓间充质干细胞(BMSC),接种于上述支架,构建细胞-支架复合体并进行成软骨诱导培养。通过电镜分析、力学测试、体内植入试验和细胞实验等检测材料纤维直径、孔径和孔隙率、力学性能,细胞在支架上生长、分化情况以及生物相容性。结果 PCL-明胶纳米纤维膜直径均匀,有较大的孔隙率和比表面积,较好的力学性能。细胞-支架共培养24 h、48 h、72 h后BMSC生长增殖良好,共培养能促进BMSC成软骨诱导分化。体内试验表明材料无毒性,对组织刺激性小,具有良好的生物相容性。结论电纺PCL-明胶纳米纤维膜有较好的力学性能、细胞亲和性和生物相容性,基本满足软骨组织工程支架条件,能够用作种子细胞承载体。Objective To construct an electrospinning polycaprolactone （PCL）/gelatin scaffold seeded with rabbit bone marrow mesenchymal stem cells （BMSCs）, and investigate its feasibility for cartilage tissue engineering. Methods PCL/gelatin （50 ： 50） nanofiber membrane as scaffold was produced by electrospinning technique. And the third generation of rabbit BMSCs was harvested and seeded on the scaffolds to develop a stem cells/scaffolds complex, subsequently cultured for chondrogenesis. Meanwhile, the material was measured for the fiber diameter, pore size and porosity by scanning electron microscopy, tested for the mechanical properties, and examined for the proliferation, differentiation and biocompatibility of the cells with the scaffolds by in vivo implantation tests and in vitro cell experiments. Results The PCL/gelatin nanofiber membrane was of uniform diameter, high porosity and specific surface area, and appropriate mechanical properties. When cutured for 24 h, 48 h and 72 h on the scaffold, the number of the cells multiplied notablely, and the chondrogenetic process was highly enhanced. The BMSCs growed and prolifered well, and was able to promote the stem cells differentiate into cartilage. In vivo tests showed that the material was non-toxic and little irritant to the tissue, hence great biocompatibility. Conclusions The electrospinning PCL/gelatin nanofibrous membrane has good mechanical properties, cell adherence and biocompatibility, thus, can be applied as the vehide in the cartilaginous tissue engineering.

关 键 词：电纺丝 聚己内酯-明胶纳米纤维膜 骨髓间充质干细胞 软骨组织工程 

分 类 号：R318.08[医药卫生—生物医学工程]