精制血府胶囊对缺氧缺糖心肌细胞ET-1与ECE-1基因表达影响的研究  

Effect of Refined Xuefu Capsule on Gene Expression of ET - 1 and ECE - 1 in Anoxic and Sugar Deficient Myocardium

作　　者：王伟[1] 史大卓[2] 陈可冀[2] 钟蓓[3] 马晓昌[2] 朱应葆[3] 

机构地区：[1]北京中医药大学东直门医院,北京100700 [2]中国中医研究院西苑医院,北京100091 [3]北京医科大学第三临床医学院,北京100029

出　　处：《中国医药学报》2000年第4期17-19,共3页China Journal of Traditional Chinese Medicine and Pharmacy

摘　　要：本研究采用原位杂交技术,探讨精制血府胶囊对与心肌缺血密切相关的基因表达的影响,阐明其抗心肌缺血的分子机制。结果:精制血府胶囊、血府逐瘀胶囊,硫氮革酮等三种“药血清”组的LDH、CK、COT释放量与同浓度的兔血清组比较均有极显著差异(p<0.01)。精制血府胶囊组、硫氮草酮组抑制LDH的释放较血府逐瘀胶囊组显著(p<0.01)。原位杂交显示:ET-1的基因杂交结果,精制血府胶囊组与正常培养细胞组均未见杂交颗粒,硫氮草酮组与缺氧缺糖组心肌细胞核周围胞质中可见大量杂交颗粒;血府逐瘀胶囊组,兔血清组仅见少量杂交颗粒。ECE-1的基因杂交结果,缺氧缺糖组,兔血清组与硫氮草酮组心肌细胞胞质中均可见大量杂交颗粒,且三组无明显差异;精制血府胶囊组、血府逐瘀胶囊组、正常培养组均未见阳性杂交信号。结果表明,精制血府胶囊具有心肌细胞保护作用,并与其抑制ET-1,ECE-1的基因表达有关。To observe the effect of refined Xuefu capsule (RXFC) on gene expression of myocardical ischemia by in situ hybridization (ISH) and explore the molecular mechanism of acting against myocardical ischemia. There was remarkable difference on the release of LDH, CK and GOT between rabbit serum group on the same concentration and medical serum group including RXFC group,XFC group and ditiazem group(p< 0.01). There had also a remarkable statistical difference between RXFC group, XFC group and ditiazem group on the inhibition of releasing IDH(p < 0.01) . ISH evidence indicated that the hybrid result of ET - 1 gene was as follows. There was no hybrid granule between RXFC group and normal culture cell group. There were plenty of hybrid granules between ditizem group and anoxic and sugar deficient group. The hybrid result of ECE-1 gene was as follows. There were plenty of hybrid granules in cytosol around myocardium kargon among anoxia and sugar deficient group, rabbit serum group and ditiazem group. There was no significant difference among these three groups. There was no positive hybrid signal among RXFC group, XFC group and normal culture group. It indicated that RXFC possess protective function on myocardium. It was related to inhibiting gene expression of ET - 1 and ECE - 1.

关 键 词：精制血府胶囊 心肌缺血 ET-1 ECE-1 基因表达 

分 类 号：R285.5[医药卫生—中药学]