三七总皂苷抗炎作用机制的实验研究  

Studies on Anti-Inflammatory Mechanisms of Saponin of Panax pseudo ginseng var notoginseng (Sanchi)

作　　者：李淑慧[1] 李晓辉[1] 楚延[2] 

机构地区：[1]第三军医大学药理教研室,重庆400038 [2]华西医科大学药理教研室

出　　处：《中草药》2000年第9期676-678,共3页Chinese Traditional and Herbal Drugs

基　　金：重庆市中医药管理局课题!No:990 32

摘　　要：以角叉菜胶 (Car) 2 5 mg/kg sc诱导大鼠气囊滑膜炎为模型 ,分别采用 L owry法、试管稀释法、硫代巴比妥酸分光光度法 ,观察了三七总皂苷 (Pn S)对气囊滑膜炎渗出液中蛋白含量、白细胞数量及脂质过氧化物丙二醛(MDA)含量的影响 ,此外 ,采用细胞色素 C还原法及放射免疫分析法 ,进一步观察了 Pn S对渗出液中中性粒细胞(Neu)释放超氧阴离子 (O÷2 )及其胞内 c AMP含量的影响。结果发现 :Pn S 6 0 ,12 0 ,2 40 m g/kg 3个剂量组均能剂量依赖性地抑制 Car诱导的白细胞游出和蛋白渗出 ;显著降低 MDA含量、抑制 Neu释放 O÷2 ;但能升高 Neu内c AMP含量。提示 Pn S对大鼠气囊滑膜炎具有明显抗炎作用 ,其抗炎机制与升高 Neu内 c AMP从而抑制氧自由基生成。Effects of saponin of Panax pseudo ginseng var notoginseng (Burk ) Hoo et Tseng (PnS) on neutrophil (Neu) count, protein and malondialdehyde (MDA) contents in the exudate of rat acute air pouch synovitis model induced by sc injection of 25 mg/kg carrageenan were investigated by Lowry and test tube dilution methods and thiobarbituric acid spectrophotometry Its effects on the intracellular cAMP content in and release of O ÷ 2 from Neu were further assessed by radioimmunoassay and cytochrome C reduction methods respectively The results showed that PnS 60,120,240 mg/kg ip reduced the migration of Neu and exudation of protein, lowered the content of MDA and inhibited the release of O ÷ 2 from Neu; but elevated the cAMP content of Neu in a dose dependent manner These results revealed that Pns has an obvious anti inflammatory effect and its primary action was related to the increase of cAMP content in Neu, followed by inhibition of the free radical production

关 键 词：三七总皂苷 气囊滑膜炎 中医药疗法 大鼠 

分 类 号：R274.967[医药卫生—中西医结合] R285.5[医药卫生—中医骨伤科学]