钙调素拮抗剂与钙通道阻滞剂对离体大鼠心脏钙反常损伤心肌保护作用的研究  

Effects of calmodulin antagonist and slow calcium channel antagonist on reperfusion injury of isolated rat hearts in calcium paradox

作　　者：宋东哲[1] 姚璐[2] 陈志周 岳远坤 孙海燕[3] 刘成玉[3] 谭润鸾[3] 陆兴熠[3] 刘丽秋[3] 

机构地区：[1]青岛大学医学院附属医院心内科,山东青岛266003 [2]青岛海军401医院,山东青岛266071 [3]青岛大学医学院诊断学教研室,山东青岛266021

出　　处：《中国病理生理杂志》2000年第9期828-830,共3页Chinese Journal of Pathophysiology

摘　　要：目的 :观察钙调素拮抗剂三氟拉嗪 (TFP)及钙通道阻滞剂异博定 (VP)对缺钙 -复钙损伤心肌的保护作用。方法 :应用TFP与VP对离体大鼠心脏进行灌流。检测心肌钙含量、灌流液中乳酸脱氢酶 (LDH)及心肌蛋白漏出量等指标。结果 :TFP显著减少损伤心肌的钙含量、LDH及蛋白漏出量 ,与对照组相比差异显著 (P <0 0 1)。VP灌注组与对照组相比各项指标差异无显著 (P >0 0 5 )。TFP与VP联合应用各项检测指标值明显低于单独应用TFP(P <0 0 1)。结论 :TFP有效减轻钙反常心肌损伤的程度 ;VP对钙反常心肌无保护作用。联合应用TFP与VP的心肌保护效果更佳。钙反常时Ca2 +大量内流主要通过钙调素介导 。AIM: To observe the protective effect of calmodulin antagonist trifluoperazine(TFP) and slow calcium channel antagonist verapamil (VP) upon calcium overload injury. METHODS: The calcium content of the myocardium, lactate dehydrogenase (LDH) release, protein loss and coronary flow were measured after the isolated rat hearts were perfused by TFP and VP. RESULTS: TFP could significantly reduce the tissue calcium , LDH release and protein loss of the myocardium ( P <0 01) while VP had no such effects.The above indexes were further reduced when TFP and VP were used simutaneously( P <0 01). CONCLUSIONS:TFP can effectively alleviate the severity of the myocardium injury during calcium paradox while VP has no such effects.The simultanous use of VP and TFP is more effective in preventing tissue damage of the heart than the use of TFP alone.The massive influx of Ca 2+ in calcium paradox is mainly a calmodulin related process.

关 键 词：心肌保护 大鼠 钙调素拮抗剂 钙通道阻滞剂 

分 类 号：R542.202[医药卫生—心血管疾病]