非创伤性预处理对大鼠缺血心肌保护效应机制的探讨  

Study on protective mechanism of non-wounded ischemic preconditioning on rat ischemia/reperfusion myocardium

作　　者：卢彦珍[1] 董传仁[2] 刘永明[2] 张友云[2] 马春艳 

机构地区：[1]长治医学院病理生理学教研室,山西长治046000 [2]湖北医科大学病理生理学教研室,湖北武汉430071

出　　处：《中国病理生理杂志》2000年第9期825-838,共14页Chinese Journal of Pathophysiology

摘　　要：目的 :探讨非创伤性下肢缺血预处理对大鼠心肌保护效应的机制。方法 :用雄性SD大鼠 36只 ,分对照、缺血再灌注、经典缺血预处理及非创伤性下肢缺血预处理 4组。分别观察以下指标 :血浆一氧化氮 (NO)水平 ;心肌热休克蛋白 70 (HSP70 )mRNA表达 ;心肌 5’ -核苷酸酶 (5’ -NT)及过氧化氢酶 (CAT)活性。结果 :经典及非创伤性下肢缺血预处理后 ,血浆NO水平显著高于缺血再灌注组和对照组 (P <0 0 1) ,心肌HSP 70mRNA表达明显增强 ,心肌 5’ -NT、CAT活性升高 (P <0 0 5 ,vsI/R) ,而经典及非创伤性下肢缺血预处理两组间上述指标无显著差异 (P >0 0 5 )。结论 :非创伤性下肢缺血预处理保护心肌的机制可能与经典缺血预处理相似 ,均是通过提高心肌内源性保护物质NO、HSP70mRNA、CAT及 5’ -NT显示预处理效应。AIM:To investigate probable protective mechanism of non-wounded legs ischemic preconditioning on ischemia/reperfusion(I/R) myocardium. METHODS: 36 male SD rats, weighting (250±30) g,were divided into 4 groups.They are normal control(NC);I/R; classical ischemic preconditioning(C-IPC)and non-wounded legs ischemic preconditioning(N-WIPC). NO in plasm,expression of myocardial HSP 70 mRNA, the activities of 5'-NT and CAT of myocardium were observed in all groups. RESULTS:The level of NO in plasm significantly enhanced in groups C-IPC and N-WIPC compared with that in groups I/R and NC ( P <0 01),expression of myocardial HSP 70 mRNA was greatly increased in both C-IPC and N-WIPC groups, the activities of 5'-NT, CAT of myocardium were also raised in groups C-IPC and N-WIPC ( P< 0 05 vs I/R),but there was no difference between C-IPC and N-WIPC( P >0 05). CONCLUSION:The possible protective mechanism involved in N-WIPC is similar to that in C-IPC, which is due to increase of endogenous myocardial protective substances.

关 键 词：大鼠 心肌缺血 缺血预处理 心肌保护 

分 类 号：R542.2[医药卫生—心血管疾病]