水稻基因组中U2snRNA基因连锁的分析  

Analysis of the Linked U2snRNA Genes in Rice Genome

作　　者：冯征宇[1] 王喜萍[2,1] 孙崇荣[2,1] 曹凯鸣[2,1] 

机构地区：[1]复旦大学生物化学系,上海200433 [2]复旦大学生命科学学院

出　　处：《复旦学报（自然科学版）》2000年第3期269-272,共4页Journal of Fudan University：Natural Science

基　　金：国家科技部水稻基因组研究计划资助项目 (10 1 0 9 0 5 )

摘　　要：根据U2snRNA基因的保守性和结构特点设计引物 ,通过PCR法扩增U2snRNA连锁基因间区的DNA片段 ,并进行顺序分析 ,证实在水稻中至少存在一组U2snRNA连锁基因 .对水稻总DNA的PCR分析结果表明 ,水稻中可能还存在其他形式的连锁基因 .PCR方法可以有效地应用于结构保守基因的连锁分析中 .A pair of primers, U2d and U2u, were designed based on the conserved region in U2snRNA gene structure. The spacer fragment between U2snRNA linked genes in recombinant DNA from rice was amplified by PCR and seguenced, demonstrating that at least one U2snRNA gene existed in rice genome. The analysis of PCR product from rice total DNA showed that other linked genes were present in rice genome. Therefore, PCR method should be applicable effectively in analysis of linked genes which possessed conserved sequences.

关 键 词：U2snRNA基因 基因连锁 PCR法 水稻 基因组 

分 类 号：S511.01[农业科学—作物学] Q343.241[生物学—遗传学]