农杆菌介导GNA基因对水稻的遗传转化  

Agrobacterium tumefaciens-Mediated Transformation of Rice for GNA Gene

作　　者：刘志学[1,2] 何艺园[3,1] 曹清玉[3,1] 王卉[3,1] 徐亚南 马向前 唐克轩 

机构地区：[1]复旦大学遗传学研究所 [2]上海大学生命科学学院,上海201800 [3]复旦大学生命科学学院

出　　处：《复旦学报（自然科学版）》2000年第3期277-281,共5页Journal of Fudan University：Natural Science

基　　金：国家科委八六三高科技资助项目 (10 1 0 10 10 1) ;洛氏基金资助课题

摘　　要：在 pCAMBIA330 0质粒中插入带有RSs 1启动子的GNA基因 ,并利用农杆菌介导的遗传转化将其导入水稻的微不定芽受体 ,得到了再生植株 .PCR ,Southernblot和Westernblot的检测结果初步证明GNA基因已经整合到水稻的基因组中并得到了表达 .GNA gene promoted by RSs 1 promoter was constructed into the pCAMBIA3300 plasmid vector, then transformed into micro adventitious bud of rice mediated by Agrobacterium tumefaciens . Regenerated plants were obtained from the transformed bud. The preliminary results of PCR, Southern blot and Western blot for the transgenic plants showed that GNA gene was introduced into the rice cells and expressed.

关 键 词：农杆菌 GNA基因 遗传转化 水稻 介导 

分 类 号：S511.035.2[农业科学—作物学] Q789[生物学—分子生物学]