Wolbachia在中国稻田飞虱中的感染和传播  

Infection and Transmission of Wolbachia in Chinese Planthopper Species

作　　者：甘波谊[1] 周伟国[2,3] 赵新燕[2,3] 冯丽冰 李昌本[2,3] 

机构地区：[1]复旦大学遗传学研究所,上海200433 [2]复旦大学生命科学学院 [3]复旦大学遗传工程国家重点实验室

出　　处：《复旦学报（自然科学版）》2000年第3期331-333,337,共4页Journal of Fudan University：Natural Science

基　　金：美国McKnight基金资助项目

摘　　要：用PCR方法检测了采集于不同地域的稻田飞虱共生菌Wolbachia的感染 ,发现灰飞虱Laodelphaxstri atellus,褐飞虱Nileparvatalugens,白背飞虱Sogatellafurcifera为Wolbachia所感染 .克隆了编码Wolbachia外膜蛋白质的wsp基因并进行了序列测定 .对wsp的RFLP分析证实了这些飞虱为单一Wolbachia感染 .还发现一种能同时寄生于这 3种昆虫的一类寄生蜂为同种Wolbachia感染 .Wolbachia可能通过这种寄生蜂在不同昆虫间横向传播 .The infection of the bacterial symbiont Wolbachia in planthoppers was detected by PCR method. Laodelphax striatellus, Nileparvata lugens and Sogatella furcifera were found to be infected with Wolbachia . The gene wsp , encoding Wolbachia outer membrane protein, was cloned and sequenced. The RFLP analysis confirmed that those planthoppers were infected with single Wolbachia stain. A parasitic wasp, which can parasitize the planthoppers, was found to be infected with the same Wolbachia . Wolbachia might transfer between different insects horizontally via this wasp.

关 键 词：WOLBACHIA 灰飞虱 褐飞虱 中国 稻田 感染 传播 

分 类 号：S435.112.3[农业科学—农业昆虫与害虫防治]