MicroRNA-29b在动脉粥样硬化血管中的表达  

MicroRNA-29b expression in atherosclerotic aorta

作　　者：伍韵贤[1,2] 曾昭华 苏子焯[1] 刘宝骅[1] 杜一鹏[1] 梁丽英[1] 

机构地区：[1]广州医学院第一附属医院心血管内科,广东广州510375 [2]广东省广州市海珠区石溪中医医院内科,广东广州510375

出　　处：《中国医药导报》2013年第10期4-5,8,共3页China Medical Herald

基　　金：国家自然科学基金资助项目(编号81170280);广东省第二批科学事业费计划项目(编号2009B030801359);广东省医学科研基金(编号9151018201000020)

摘　　要：目的应用荧光定量PCR技术分析MicroRNA-29b(miR-29b)在Apo-E基因敲除小鼠的动脉粥样硬化(atheroscle-rosis)模型斑块血管中的表达情况,探讨miR-29b在动脉粥样硬化发生、发展过程中的作用。方法 8只5周龄、雄性SPF级载脂蛋白E基因敲除小鼠(C57BL/6J-ApoE-/-,apolipoprotein E-deficient mice)为实验组(FS组)。8只雄性、SPF级、相同周龄的C57BL/6J小鼠为对照组(NS组)。高脂(1%胆固醇+15%猪油)喂食15、20周龄取材检测。结果 FS组和NS组体重[(29.10±2.53)g和(33.20±5.18)g]无明显差异(P>0.05);FS组较NS组血清总胆固醇[(13.52±1.58)mmol/L和(2.12±0.35)mmol/L]显著增高(P<0.01);FS组主动脉出现典型的无动脉粥样硬化斑块;NS组主动脉管壁正常,无动脉粥样硬化斑块;FS组主动脉弓miR-29b的表达较NS组[平均吸光度值:(2.10±0.27)和(17.56±1.07)]明显降低(P<0.01)。结论在高脂喂食的载脂蛋白E基因敲除小鼠动脉粥样硬化模型中,miR-29低表达可能与动脉粥样硬化斑块的形成有关。Objective To determine the role and relationship of MicroRNA-29b （miR-29b） in the development process with the atherosclerotic were used fluorescence quantitative PCR technical in the expression of miR-29b on atherosclerotic aorta in Apolipoprotein E-deficient mice. Methods 8 five-week-old male mice which were knocked out apo-E gene grade of SPF C57BL/6J were grouped as the experiment group （FS group）. 8 male mlce of the same age grade of SPF C57BL/6J were grouped as the control group （NS group）. Two groups of mice were fed up with high fat diet for fifteen weeks （1%cholesterol＋15%lard）. When the mice were twenty weeks, took some samples and did the test. Results There was no significant difference between FS group and NS group in the body weight [（29.10±2.53） g, （33.20±5.18）g]（P 〉 0.05）. Serum total cholesterol was significantly higher in the FS group than that in the NS group [（13.52±1.58）mmol/L, （2.12±0.35） mmol/L] （P 〈 0.01）. There were typical atherosclerotic plaque in aorta in the FS group, but they were normal and there were no atherosclerotic plaque in the NS group. The expression of miR-29b in aortic arch was significantly lower in the FS group than that in the NS group [IOD： （2.10±0.27）, （17.56±1.07）] （P 〈 0.01）. Conclusion The forming of atherosclerotie plaque in atherosclerosis model which Apolipoprotein E-deficient mice were fed with high fat diet concern to the lower expression of miR-29b.

关 键 词：MicroRNA-29b 载脂蛋白E基因敲除小鼠 动脉粥样硬化 

分 类 号：R543.4[医药卫生—心血管疾病]