应用树脂铸型法研究木质部导管的内部结构  

APPLICATION OF THE SESIN-CASTING METHOD TO THE STUDY OF THE VESSEL LUMENS OF XYLEM

作　　者：张秀军 孟丽军 樊拥军[2] 罗立新 吴树明[2] 

机构地区：[1]唐山师范专科学校生物系和唐山高等专科学校化工系,唐山063000 [2]南开大学生物系,天津300071

出　　处：《南开大学学报（自然科学版）》2000年第2期121-123,共3页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：国家自然科学基金!(39670 0 4 6)资助项目

摘　　要：将树脂铸型法 ( resin casting method)用于藤本和灌木共五种植物导管的内部结构研究 .干燥的小木块用苯乙烯包埋 ,完全聚合后 ,用过氧化氢 /冰醋酸 ( 1∶ 1 )溶液和浓硫酸溶液交替处理 ,完全去除细胞壁成分 ,得到的导管树脂铸型在扫描电子显微镜下观察 ,能清晰、精确地反映出纹孔道、纹孔室、螺纹加厚、导管分子端壁及尾等三维立体结构特征 .The vessel lumens of five species belong to shrubs and lianas were studied by applying a resin casting method. Dry blocks from stems were embedded in polystyrene and then cell wall were completely removed by alternate and repeated treatments with hydrogen peroxide/ice acetic acid solution and sulphuric acid. Long casts of vessels and vessel elements were easily separated. These can be examined with light microscope or dissecting microscope. Casts have such high fidelity and high resolution that details of pits canals, pits chambers, helical thickness, vessel tips, trials and perforation plates can be studied.

关 键 词：树脂铸型法 木质部 导管 内部结构 藤本 灌木 

分 类 号：Q944.66[生物学—植物学]