枸杞子对人宫颈癌Hela细胞生长的影响  

Effects of Fructus lycii on Growth of Cervical Cancer Hela Cells

作　　者：欧嘉文[1] 何冬梅[2] 李江滨[1] 余涌珠[1] 郑若洋[1] 陈婉嫦[1] 杨丽梅[1] 

机构地区：[1]广东医学院医学检验学院,广东东莞523808 [2]惠州市中医医院妇产科,广东惠州516001

出　　处：《中国食物与营养》2013年第3期69-71,共3页Food and Nutrition in China

基　　金：全国高等学校特色专业建设点"医学检验"(项目编号:TS10466)

摘　　要：目的:体外实验探讨枸杞子对人宫颈癌细胞(Hela)生长的影响。方法:不同浓度的枸杞子水煎液处理Hela细胞,采用细胞贴壁率实验测定细胞的粘附能力,通过细胞划痕实验观察细胞的迁移能力、利用细胞增殖实验检测细胞增殖抑制率,运用流式细胞术分析细胞周期变化。结果:与对照组相比,5.0mg/mL枸杞子水煎液处理后的Hela细胞贴壁率减少(P<0.01),细胞迁移能力减弱(P<0.01),对Hela细胞有显著的抑制作用,抑制率最高达84.88%(P<0.01),且呈现时效和量效关系;流式细胞术结果表明,枸杞子水煎液组对Hela细胞有一定的S期阻滞作用。结论:枸杞子能有效抑制人宫颈癌细胞的增殖、粘附和迁移能力,并改变细胞的周期分布。[Objective ] To investigate the effects of Fructus lycii on the in-vitro growth of cervical cancer Hela cells. [Method ] He- la cells were treated with different concentrations of the decoction of Fructus lycii, and its effects on the adhesion and migration ability of Hela cells were determined by the cell-matrix adhesion assay and wound healing. The cell proliferation assay and Flow Cytometer were used to detect the inhibitory effects and the change of growing cycle. [Result ] Compared with control groups, the adhesive ratio of Hela cells treated with 5.0mg/mL Fructus lycii reduced （ P 〈 0. 01 ） and the migration ability of Hela cells became weaker （ P 〈 0. 01 ） . The cell proliferation assay showed that Fructus lycii inhibited the growth of Hela cells effectively and the inhibited radio reached up to 84. 88% （P 〈 0. 01 ）, and the inhibit function of Fructus lycii to Hela cells showed an obvious relationships of time-effectiveness and does-effec- tiveness. The result of flow cytometry showed that Fructus Lycii could block the cell cycle at S phase. [Conclusion ] Fructus lycii could in- hibit the ability of adhesion, migration and proliferation of Hela ceils, and it also changed the cycle of the Hela cells.

关 键 词：枸杞子 宫颈癌 HELA细胞 细胞生长 

分 类 号：R285.5[医药卫生—中药学]