丹参水提物预防糖皮质激素对大鼠睾丸损害的实验研究  

Experimental Study of Danshen Aqueous Extracts for Preventing Testicle Lesions Damages Induced by Glucocorticoid in Rats

作　　者：周乐[1] 崔燎[1] 吴铁[1] 罗世英[1] 

机构地区：[1]广东医学院药学院,东莞市523808

出　　处：《广西医学》2013年第3期275-277,共3页Guangxi Medical Journal

基　　金：广东医学院面上项目(XK1002)

摘　　要：目的观察丹参水提物对醋酸泼尼松致大鼠睾丸结构和功能损害的预防作用。方法 3月龄雄性SD大鼠32只按随机数字表法分为4组,每组8只。正常对照(Cont)组给予双蒸水灌胃,醋酸泼尼松(GC)模型组给予醋酸泼尼松加双蒸水灌胃,丹参高剂量(DSH)组给予醋酸泼尼松加丹参水提物5 g/kg灌胃,丹参低剂量(DSL)组给予醋酸泼尼松加丹参水提物2.5 g/kg灌胃,各组大鼠均灌胃给药90 d。测量睾丸重量和睾丸内脏指数;观察睾丸形态学;放射免疫法检测血清睾酮含量。结果 (1)大体病理:各组大鼠睾丸重量、睾丸内脏指数比较,差异无统计学意义(P>0.05)。(2)形态学:醋酸泼尼松组大鼠睾丸生精小管和生精细胞均萎缩、数目减少,间质纤维化增生;DSH组和DSL组均可见生精小管数量增加,体积增大,且生精小管肥大,功能增强,间质纤维化减少。(3)血清睾酮含量4组比较,差异有统计学意义(P<0.05),DSL组含量最高,其次为DSH组、Cont组、GC组(P<0.05)。结论丹参水提物可以预防糖皮质激素所致的大鼠睾丸结构损害和功能改变。Objective To observe the prevention effects of Danshen aqueous extracts on prednisone acetate-induced damages to structure and function of testicle in rats. Methods Thirty-two male SD rats of 3 month old were divided into four groups by random digits table,eight rats in each group. The control rats（Cont group） were treated intragastrically with double-evaporated water. In the other three groups, the rats were administrated intragastrically with prednisone acetate and double-evaporated water（GC group）,with prednisone acetate and Danshen aqueous extracts with the dose of 5 g/kg（DSH group） ,and with prednisone acetate and Danshen aqueous extracts with the dose of 2.5 g/kg（DSL group） ,respectively,the rats in each group were treated with intragastric administrated for 90 days. Both weight of rat testicle and index of important organ were measured,and testicle histomorphology was observed. The serum testosterone was detected by radioimmunoassay. Results ① Organism pathology：The weight of rat testicle and index of important organ showed no significant difference among all groups（P 〉 0.05）. ② Morphology：Both seminiferous tubules and spermatogenic cells were cataplastic, cell numbers were reduced, and the fibrosis of intercellular substance was found in GC group. In DSH group and DSL group, numbers and volumes of seminiferous tubules and spermatogenic cells were increased and the hypertrophy and the function improvement of seminiferous tubules were observed. Moreover, interstitial fibrosis was decreased. ③ Sermn testosterone level showed significant difference among four groups（P 〈0. 05） ,which was in sequence of DSL group 〉 DSH group 〉 Cont group 〉 GC group, and the highest was in DSL group （ P 〈 0.05 ）. Conclusion Danshen aqueous extracts can prevent damages to testicle structure and function changes induced by glucocorticoid in rats.

关 键 词：丹参水提物 醋酸泼尼松 睾酮 睾丸 大鼠 

分 类 号：R697[医药卫生—泌尿科学] R-332[医药卫生—外科学]