福尔马林口服剂量对绵羊瘤胃微生物群落的影响  

Effects of Oral Administration with Different Doses of Formalin on the Rumen Microbiocoenosis in Sheep

作　　者：栗志坚[1] 雒秋江[1] 上官京[1] 李海英[1] 李昊[1] 

机构地区：[1]新疆农业大学动物科学学院动物营养实验室,乌鲁木齐830052

出　　处：《新疆农业大学学报》2013年第1期1-6,共6页Journal of Xinjiang Agricultural University

基　　金：新疆维吾尔自治区动物营养与饲料科学重点学科经费资助

摘　　要：选取4只1.5岁龄、体重约35kg安装了永久性瘤胃瘘管的小尾寒羊公羊,采用4×4拉丁方设计,在精粗比为1∶1的日粮条件下,研究口服"有利"剂量(1.0mL/kg日粮)和"不利"剂量(3.0和4.0mL/kg日粮)福尔马林时瘤胃微生物群落的变化。结果表明,绵羊分别口服福尔马林0,1.0,3.0,4.0mL/kg日粮,其瘤胃液细菌总数分别为(120.69×109±17.49×109),(191.11×109±20.27×109)(P<0.01),(94.56×109±13.01×109)(P<0.05),(79.60×109±12.89×109)(P<0.01)CFU/mL;原虫总数分别为(9.85×105±0.66×105),(5.02×105±0.56×105)(P<0.01),(1.70×105±0.36×105)(P<0.01),(1.13×105±0.36×105)(P<0.01)CFU/mL;瘤胃液平均氨态氮浓度分别为(25.84±6.65),(21.57±6.07)(P<0.01),(15.54±4.41)(P<0.01),(8.91±3.52)(P<0.01)mg/100mL,而日粮自由采食量分别为(2078.4±107.0),(2284.4±55.6)(P<0.01),(1965.8±53.4)(P<0.01),(1854.5±44.9)(P<0.01)g/d。本研究表明,口服福尔马林使绵羊瘤胃液原虫数量减少、氨态氮浓度降低,但在"有利"剂量时,瘤胃细菌总数和自由采食量增加,而在"不利"剂量时,瘤胃细菌总数和自由采食量则降低。瘤胃细菌数量的增减是口服福尔马林产生有利还是不利作用的决定因素。In this study,the effect of the formalin at the beneficial （1.0 mL/kg daily diet）and adverse （3.0 and 4.0 mL/kg）doses on rumen microbiocoenosis was studied. Four small Tail Han-sheep ram with the body weight of 35 kg,1.5-year-old,and with the rumen syrinx,were used with the 4X4 Latin square, fed a diet of concentration （50~） and forage （50%）. The results showed that when the sheep oral adminis- tration of formalin with the concentration at 0,1.0,3.0 and 4.0 mL/kg diet,the total number of rumen bacteria was （120. 69 X109 _＋ lT. 49X10~）,（191.11X109~20.27X109） （P %0. 01） , （94. 56 X109 ＋13. 01 X109） （P %0.05） and （79.60X109~12.89X109） （P %0.01）CFU/mL respectively;the total number of rumen protozoa was （9.85X10s^0.66X10S）,（5.02X10S_＋0.56X10s） （P %0.01）,（1.70N10S^0.36X l0s） （P %0.01）and （1.13N10s ~0.36X10s） （P %0.01）CFU/mL,respectively;the average concentration of rumen ammonia nitrogen was （25.84~6.65）,（21.57-~6.07） （P %0.01）, （15.54-＋4.41） （P %0.01）, （8.91_＋3.52） （P%0.01） mg/100mL,respectively^the voluntary intake of DM was （2078.4_--t-107.0）, （2284.4-＋55.6） （P %0.01） ,（1965.8_＋53.4） （P %0.01）, （1854.5-＋44.9） （P %0.01）g/d respectively. This study showed that oral administration of formalin decreased the number of protozoa,the ammonia ni- trogen concentration decreased,but at beneficial dose,the total tureen bacterial and voluntary intake were increased , and at adverse dose , they were decreased. It is concluded that the increase or decrease of rumen bacteria is decisive factor for determining if the oral administration of formalin is beneficial or adverse.

关 键 词：绵羊 福尔马林 瘤胃 微生物群落 

分 类 号：S826.5[农业科学—畜牧学]