循环游离DNA在多发性肌炎和皮肌炎中的作用  

Contribution of circulating cell-free DNA in patients with polymyositis and dermatomyositis

作　　者：张思功[1,2] 卢昕[2] 田小兰[2] 王艳[2] 杨文芳[2] 张学智[2] 王国春[1,2] 

机构地区：[1]北京协和医学院研究生院,北京100073 [2]中日友好医院风湿免疫科,北京100029

出　　处：《中日友好医院学报》2013年第2期72-75,共4页Journal of China-Japan Friendship Hospital

基　　金：国家自然科学基金资助项目(81172860);北京市科委资助项目(D101100050010018)

摘　　要：目的:研究循环游离DNA(cfDNA)在多发性肌炎(PM)和皮肌炎(DM)中的作用。方法:使用PicoGreen试剂盒检测61例PM/DM患者、34例系统性红斑狼疮(SLE)患者和48例健康对照的血浆cfDNA浓度,并与患者的血清学指标做相关性分析。结果:cfDNA浓度在PM/DM组是247.1±61.09ng/ml,显著高于健康对照组(197.1±31.36ng/ml,P<0.01),显著低于SLE组(281.9±82.21ng/ml,P<0.01)。cfDNA与血沉和血清球蛋白呈显著正相关(P<0.01,P<0.05),与C3呈显著负相关(P<0.01)。结论:cfDNA可能与PM/DM疾病活动度相关。Objective：To investigate concentration of plasma circulating cell-free DNA（cfDNA）in patients with polymyositis（PM）and dermatomyositis（DM）.Methods：Sixty one patients with PM/DM ,34 patients with systemic lupus erythematosus （SLE）and 48 age-and sex-matched healthy controls were included in a retrospective, cross-sectional study.The efDNA concentration was measured with PicoGreen Kit.Correlation analysis were performed between cfDNA concentration and other serological markers.Results：efDNA in PM/DM group was 247.1±61.09ng/ml,significantly higher than that in healthy control group （197.1±31.36ng/ml,P〈0.01）and significantly lower than that in SLE group （281.9±82.21ng/ml,P〈0.01）.cfDNA positively correlated with erythroeyte sedimentation rate （P〈0.01）and serum globulin （P〈0.05）, and inversely correlated with C3 （P〈0.01）.Conclusion ： Elevated level of efDNA are involved in PM/DM and may correlate with PM/DM disease activity.

关 键 词：多发性肌炎 皮肌炎 循环游离DNA 

分 类 号：R593.26[医药卫生—内科学]