牙本质非胶原蛋白对人牙髓干细胞增殖活性及矿化能力的影响  

Effects of dentin non-collagenous proteins on the proliferation and mineralization of human dental pulp stem cells

作　　者：蒋玉姣[1] 曹灵[1] 俞艳[1] 王利娟[1] 于金华[1] 张光东[1] 

机构地区：[1]南京医科大学口腔医学研究所.附属口腔医院牙体牙髓病科,江苏南京210029

出　　处：《口腔生物医学》2013年第1期15-18,共4页Oral Biomedicine

基　　金：江苏省自然科学基金(BK2009346)

摘　　要：目的:明确牙本质非胶原蛋白(dentin non-collagenous proteins,dNCPs)对人牙髓干细胞(human dental pulp stem cells,hDPSCs)增殖及矿化能力的影响。方法:通过细胞活性噻唑蓝(MTT)比色法、碱性磷酸酶(ALP)活性检测、茜素红钙化结节染色和氯代十六烷基吡啶定量分析钙离子浓度,检测10μg/mL dNCPs对hDPSCs增殖和矿化能力的影响。结果:dNCPs分别诱导1、3、5、7、9d,人牙髓干细胞增殖活性与对照组相比无显著性差异(P>0.05);诱导3、5、7d时,人牙髓干细胞ALP活性明显上调,与对照组相比有统计学差异(P<0.01);诱导2周后,茜素红染色显示10μg/mL dNCPs组出现较大钙化结节,定量分析显示,其形成钙化结节的能力明显高于对照组(P<0.001)。结论:10μg/mL dNCPs可明显促进人牙髓干细胞的矿化能力,对其增殖活性的影响则不明显。Objective：To identify the effects of dentin non-collagenous proteins （dNCPs） on the proliferation and mineralization of human dental pulp stem cells （hDPSCs） in vitro. Methods：HDPSCs were treated with 10 μg/mL dNCPs in normal medium for certain time periods. Proliferation of hDPSCs was evaluated by MTT eolorimetric assay. Alkaline phosphotase （ALP） activity was determined by enzymekinetics methods. Alizarin red staining and cetylpyridinium chloride （CPC） assay were performed to investigate the mineraliztion ability of hDPSCs. Results： There was no difference between the proliferation activity of hDPSCs treated with dNCPs and the control group （ P 〉 0.05 ）. ALP activity of dNCPs treated group was signigicantly upregulated （ P 〈 0.01 ）, and the formed calcified nodules of dNCPs treated group was significantly increased（P 〈0. 001 ）. Conclusions： 10 μg/mL dNCPs significantly promotes the mineralization of hDPSCs ,while the proliferation ability shows no significant difference.

关 键 词：牙本质非胶原蛋白 人牙髓干细胞 增殖 矿化 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]