富血小板纤维蛋白对兔脂肪干细胞成骨分化的影响  

Effects of platelet-rich fibrin on osteogenic differentiation of the rabbit adipose-derived stem cells

作　　者：徐炜[1] 王明国[2] 马锋[2] 杨世茂[2] 林夏莲[1] 于彦领[1] 张新新[1] 

机构地区：[1]潍坊医学院口腔医学院,山东潍坊261053 [2]济南市中心医院口腔科,山东济南250013

出　　处：《口腔生物医学》2013年第1期19-22,共4页Oral Biomedicine

摘　　要：目的:体外分离培养兔脂肪干细胞(adipose-derived stem cells,ADSCs),鉴定其分化能力并观察富血小板纤维蛋白(platelet-rich fibrin,PRF)对ADSCs成骨分化的影响。方法:取新西兰兔腹股沟处的脂肪组织,将其分离获得脂肪干细胞,培养至第三代用于实验。分别以油红O、茜素红染色鉴定其成脂和成骨分化能力。取兔耳中央动脉血一次离心法制备PRF膜。将脂肪干细胞分为2组:对照组不含PRF膜;实验组含PRF膜。用碱性磷酸酶(ALP)试剂盒检测不同时间点PRF对ADSCs成骨分化的影响。结果:脂肪干细胞呈长梭形贴壁生长;油红O及茜素红染色均呈阳性;在不同的时间点,实验组ALP活性值均高于对照组(P<0.05)。结论:ADSCs具有成骨的潜能,且PRF可以促进ADSCs向成骨细胞分化。Objective：To separate and cultivate rabbit adipose-derived stem cells （ADSCs）, identify its differentiation acti,rity and observe the effects of platelet-rieh fibrin （ PRF ） on the osteogenic differentiation of these cells. Methods： Inguinal fat tissue extracted from New Zealand rabbits and isolated adipose derived stem cells. The third generation ADSCs were used in the experiment. The methods of oil red Ostaining and alizarin red staining were carried out to identify the a dipogenie and osteogenic differentiation. Blood collection was carried out from central artery of the rabbit and obtain the PRF membrane. The ADSCs were divided into two groups ： the experiment group with a PRF membrane ;the control group without a PRF membrane. The alkaline phosphatase kit was used to observe the effect of PRF on the osteogenic differentiation of ADSCs. Results： The rabbit ADSCs grew in the way of long spindle adherence. The resuhs of both oil red Ostaining and alizarin red staining of the cells were positive. At different time points, the expression of alkaline phosphatase in the experiment group was significantly higher than that in the control group （ P 〈 0.05 ）. Conclusions ： ADSCs can induced osteogenie differentiation and PRF can promote ADSCs to differentiate into osteoblasts.

关 键 词：脂肪干细胞 富血小板纤维蛋白 成骨分化 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]