南四湖河蟹养殖区气单胞菌的分离鉴定及胞外酶分析  

Separation, Identification and Extracellular Enzyme Analysis of Aeromonas in the Farming Area of Crab in Southern Four Lakes

作　　者：王宁[1] 周晶[2] 沙未来[2] 王玉芹[2] 

机构地区：[1]山东省烟台市芝罘区科技情报所,山东烟台264000 [2]曲阜师范大学生命科学学院,山东曲阜273165

出　　处：《畜牧与饲料科学》2013年第3期5-7,9,共4页Animal Husbandry and Feed Science

基　　金：国家级大学生创新创业训练计划项目(201210446007);山东省自然科学基金(ZR2010CL014);山东省高等学校科技计划项目(J12LE02);曲阜师范大学青年基金(XJ201107;XJ201001)

摘　　要：细菌分泌的明胶酶、蛋白酶、淀粉酶、磷脂酶等均属于胞外酶,在细菌致病机理中可以和溶血毒素一起进行协同作用,分解并破坏宿主组织成分,对河蟹的生长不利。为了研究南四湖河蟹养殖区水体中气单胞菌胞外产酶的情况,对该养殖区的水体微生物进行了初步筛选和分离,得到了气单胞菌菌株。该菌产胞外酪蛋白酶、淀粉酶、磷脂酶、明胶酶和溶血素,不产胞外脲酶。Gelatinase,protease,amylase and phospholipase that were secreted by bacteria were all cell extracellular enzymes, which could conduct synergistic reaction with the hemolytic toxin in the bacterial pathogenesis. They could also decompose and destroy the tissue components of hosts, which was unfavorable for the growth of crab. In order to study the production situations of extracellular enzymes from Aeromonas in the water body of crab farming area in Southern Four Lakes, microorganisms in water body of this farming area were preliminarily separated and screened to obtain Aeromonas strain. This strain could produce extracellular casein protease,amylase,phospholipase and gelatinase and hemolysin outside , but it could not extracellular urease.

关 键 词：气单胞菌 筛选鉴定 胞外酶分析 河蟹养殖区 

分 类 号：Q93[生物学—微生物学]