库拉索芦荟组织培养褐化现象的抑制研究  

Study on Browning Control in Tissue Culture of Aloe barbadensis

作　　者：代建丽[1] 宋婵婵[1] 徐晨[1] 张国彬[1] 

机构地区：[1]武昌理工学院生命科学学院,湖北武汉430223

出　　处：《安徽农学通报》2013年第6期23-24,共2页Anhui Agricultural Science Bulletin

摘　　要：褐化是芦荟组织培养中的普遍现象,严重影响组织培养的效果。以库拉索芦荟的茎段和叶片为外植体,研究了5种防褐剂活性炭、硝酸银、维生素C、L-谷氨酰胺和聚乙烯吡咯烷酮对愈伤组织诱导中褐化现象的抑制作用。结果表明,5种防褐剂都具有抑制外植体褐化的作用,其中15mg/L的AgNO3是最合适的防褐剂,其外植体褐化程度最轻,愈伤组织诱导率较高且愈伤组织生长良好。Browning is an common phenomenon in tissue culture of Aloe which affect the success.Five antibrown ing agents such as activated carbon,AgNO3,Vc,L-Gln and PVP were used to study their effects on browning con trol in tissue culture of Aloe barbadensis with stem and leaf explants.Results showed that all of the antibrown ing agents could inhibit browning and AgNO3 was the best antioxidant which has the lowest browning and high callus induction rate.

关 键 词：库拉索芦荟 愈伤组织 褐化 防褐剂 

分 类 号：Q949.718.23[生物学—植物学]