亨廷顿蛋白相关蛋白1在脊髓发育中的表达变化  

The Expressions of HAP1 in the Development of Rat Spinal Cord

作　　者：李秀凤[1] 管英俊[2] 

机构地区：[1]山东省临朐县人民医院病理科,262600 [2]山东省潍坊医学院组织胚胎学教研室,261042

出　　处：《中华全科医学》2013年第4期502-503,F0003,共3页Chinese Journal of General Practice

摘　　要：目的观察亨廷顿蛋白相关蛋白1(huntingtin-associated protein 1,HAP1)在正常大鼠脊髓发育过程中的表达变化。方法采用免疫组织化学ABC法。结果免疫组织化学结果显示:在胚胎期HAP1阳性细胞主要分布在脊髓腹侧,背侧数量较少;随着胚龄的增加,HAP1阳性细胞在脊髓灰质中分布广泛,腹侧阳性细胞体积较小,分布较密集,背侧阳性细胞体积较大,分布较稀疏。在生后早期HAP1阳性细胞呈蝶形广泛分布在脊髓灰质内,阳性细胞体积较小,分布比胚胎期稀疏;在成年期HAP1阳性细胞主要分布在脊髓背角灰质内,背角浅层的HAP1阳性细胞体积较小,数量较少,细胞为圆形,越靠近深层阳性细胞数量越少,HAP1阳性细胞有明显的胞体和突起,胞体为圆形或椭圆形,突起细长。结论 HAP1的表达贯穿脊髓发育过程中,HAP1的表达随着胚龄的增加而发生规律性变化;生后大鼠脊髓内的HAP1阳性细胞分布与脊髓灰质的分化一致。Objective To observe the expressions of HAP1 in spinal cord of normal developing rat.Methods ABC method of immunohistochemistry was used in the present study.Results During the embryonic rat,the HAP1 positive cells were mainly distributed in the ventral horn of spinal cord.The HAP1 positive cells in the dorsal horn of spinal cord in number were less than those of the ventral horn.During the development of spinal cord,HAP1 was expressed extensively in the gray matter of spinal cord.In the ventral,the HAP1 positive cells in size were small and distributed intensively.In the dorsa horn,the HAP1 positive cells in size were large and in area were less than that of the ventral horn.The expression of HAP1 in the spinal cord after birth,the HAP1 positive cells were wide spreadly distributed in the gray matter of spinal cord as butterfly.The HAP1 positive cells in size were small.But the distributed area of spinal cord in this period was less than that in embryonic period.In the adult rat,the HAP1 positive cells were mainly located in the gray matter of doral horn of spinal cord.The HAP1 positive cells had obvious cell body and processes.The processes of positive cells were thin and long.Conclusion The expressions of HAP1 had some regularity in the developing spinal cord of normal rat.In the spinal cord of postnatal rats,the distribution of the HAP1 positive cells was correlated to the differentiation of the gray matter in the spinal cord.

关 键 词：亨廷顿蛋白相关蛋白1 脊髓 发育 表达 

分 类 号：R349.63[医药卫生—基础医学] R338