呼吸道合胞病毒性毛细支气管炎患儿外周血CD4^+CD25^+Foxp3^+调节性T细胞检测及价值探讨  

Significance of CD4^+CD25^+Foxp3^+ regulatory T cells in peripheral blood of infants with respiratory syncytial virus bronchiolitis

作　　者：钱烘云[1] 李琳[1] 李朝晖[1] 姚建军[1] 张满燕[1] 

机构地区：[1]绍兴第二医院儿科,浙江绍兴312000

出　　处：《中华危重症医学杂志（电子版）》2013年第1期17-20,共4页Chinese Journal of Critical Care Medicine:Electronic Edition

摘　　要：目的探讨CD4+CD25+Foxp3+调节性T细胞(Tregs)在呼吸道合胞病毒(RSV)性毛细支气管炎患儿中的临床价值。方法采集24例RSV性毛细支气管炎组患儿、28例普通肺炎组患儿及18例健康对照组婴幼儿的外周血标本,采用流式细胞仪三色法检测外周血CD4+CD25+Foxp3+Tregs的含量,并加以比较和分析。结果毛细支气管炎组患儿外周血CD4+CD25+Foxp3+Tregs百分率(10.2±1.2)%,显著低于普通肺炎组(11.5±1.5)%及健康对照组(12.7±2.1)%,差异具有统计学意义。此外,普通肺炎组患儿外周血的CD4+CD25+Foxp3+Tregs百分率亦明显低于健康对照组,差异有统计学意义。结论 RSV性毛细支气管炎患儿外周血CD4+CD25+Foxp3+Tregs含量显著下降,从而导致免疫失衡,这很有可能是该类疾病的病理生理过程及其后演变的分子生物学机制之一。Objective To investigate the significance of CD4＋CD25 ＋Foxp3 ＋ regulatory T cells （F regs） in infants with respiratory syncytial virus （RSV） bronchiolitis. Methods Twenty-four infants with RSV bronchiolitis, twenty-eight infants with non-RSV pneumonia and eighteen healthy infants were enrolled. The percentages ofCD4＋CD25 ＋Foxp3 ＋ T regs in peripheral blood were detected by flow cytometer and analyzed among three groups. Results The percentage of CD4＋CD25 ＋Foxp3 ＋ T regs was （10.2 ± 1.2）% in infants with RSV bronchiolitis, which was markedly lower than those in infants with non-RSV pneumonia （11.5± 1.5）% and in the healthy infants （12.7 ±2.1）% （P 〈 0.05）. Furthermore, the percentage of CD4＋CD25 ＋Foxp3 ＋ T regs in infants with non-RSV pneumonia was also lower than that in healthy infants, with statistically significant difference. Conclusions The percentage of CD4＋CD25＋Foxp3＋ T regs markedly reduced in peripheral blood in infants with RSV bronchiolitis. The immune dysfunction resulting from the change of T regs may be one important factor of the pathogenesis and evolution of RSV bronchiolitis.

关 键 词：呼吸道合胞病毒 毛细支气管炎 调节性T细胞 免疫 

分 类 号：R725.6[医药卫生—儿科]