中药罗勒胶囊对大鼠移植性肝癌TACE后VEGF和Bcl-2表达的影响  

Effects of Basil Capsules on Expressions of VEGF and Bcl-2 for Transplanted Liver Cancer in rats after TACE

作　　者：冯兵[1,2,3] 朱莹[2,3] 贺嵩敏[2,3] 朱亚珍[2] 郑广娟[2,3] 

机构地区：[1]广州中医药大学第二临床医学院,广东广州510006 [2]广东省中医院中药药理实验室,广东广州510120 [3]广东省中医药科学院中药药理实验室,广东广州510006

出　　处：《南京中医药大学学报》2013年第2期146-150,共5页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：国家自然科学基金(81202960/H2810);广东省中医药局科研项目(20122144)

摘　　要：目的观察罗勒胶囊对大鼠移植性肝癌经肝动脉化疗栓塞术(TACE)后细胞中VEGF和Bcl-2表达的影响,探讨罗勒胶囊对肝肿瘤TACE后乏氧微环境的影响及意义。方法建立大鼠移植性肝癌模型,10d后将造模成功的大鼠随机分为5组。对TACE+生理盐水组和TACE+罗勒胶囊高、中、低剂量组4组大鼠进行TACE,并分别给予生理盐水10mL/(kg.d)和罗勒胶囊300、150、75mL/(kg.d)ig,对非TACE对照组大鼠只进行开腹操作,不进行TACE。给药10d后取出移植性肝癌病理标本,免疫组化法检测肿瘤组织中VEGF和Bcl-2表达情况。结果瘤质量:行TACE的4组大鼠与非TACE组比较均明显减轻(P<0.05,P<0.01),TACE+罗勒胶囊高剂量组较TACE+生理盐水组显著减轻(P<0.05);VEGF和Bcl-2阳性表达率:TACE+生理盐水组与非TACE组比均显著升高(P<0.01);TACE+罗勒胶囊高、中、低剂量组较TACE+生理盐水组显著降低(P<0.01);TACE+罗勒胶囊高剂量组较TACE+罗勒胶囊中、低剂量组显著降低(P<0.05,P<0.01)。结论中药罗勒胶囊能改善肝肿瘤局部的乏氧微环境并促进细胞凋亡,使肿瘤组织中VEGF和Bcl-2表达降低,抑制大鼠移植性肝癌TACE术后肿瘤的侵袭和转移。OBJECTIVE To observe the effects of Basil capsules on expressions of VEGF and Bcl-2 for transplanted liver cancer in rats after transeatheter arterial chemoembolization （TACE） and to research the influence and significance of Basil capsules on hypoxia environment after TACE of rats hepatoma. METHODS Transplanted hepatoma models of rats were estab- lished. Ten days later, the successful models were divided into five groups randomly. The group with TACE and saline （A）, group with TACE and high-dose Basil capsules （B）, group with TACE and moderate-dose Basil capsules （C）, group with TA- CE and low-dose Basil capsules （D） were injected with 10 mL/kg/d saline, 300 mg/kg/d/ Luole capsule, 150 mg/kg/d Basil capsule and 75 mg/kg/d Basil capsules respectively. The fifth group （E） as control group was operated just with iaparotomy. After ten days of administration, the pathological specimen of transplanted liver cancer was taken out and the expressions of VEGF and Bcl-2 in tumor tissues were detected through immunohistochemistry. RESULTS As for the tumor weight, group A, B, C and D were obviously smaller than that of group E （P〈0.01, P〈0.05） ; group B was remarkably lighter than that of group A （P〈0.05）. As for the positive expression rate of VEGF and Bcl-2, both group A and E obviously increased （P〈0. 01） ;groups B, C and D visibly deceased compared with that of group A（P〈0.01） ; groups B was remarkably lower than those of groups D and E （P〈0.01,P〈0.05）. CONCLUSION Basil capsules can improve hypoxia environment in the local part of rat hepatoma, promote cell apoptosis, depress the expression of VEGF and Bel-2 and inhibit the invasion and metastasis of tumor after TACE.

关 键 词：罗勒胶囊 大鼠移植性肝癌 经肝动脉化疗栓塞术 血管内皮生长因子 凋亡抑制基因Bcl-2 

分 类 号：R285.5[医药卫生—中药学]