影响ApoE^(-/-)小鼠动脉病理变化因素的探讨  

Discussion on the Factors Affect Pathogenic Changes of ApoE^(-/-) Mice Artery

作　　者：陶文剑[1,2] 詹冬梅[1] 艾炳蔚[3] 

机构地区：[1]南京中医药大学第二临床医学院,江苏南京210023 [2]南京医科大学第一附属医院,江苏南京210029 [3]南京中医药大学附属医院,江苏南京210029

出　　处：《南京中医药大学学报》2013年第2期162-164,197,共4页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：江苏省卫生厅"六大人才高峰"资助项目(2011)

摘　　要：目的探讨不同脂肪和胆固醇含量饲料喂养对于早期ApoE-/-小鼠动脉粥样硬化进展变化的影响。方法 8只6周龄ApoE-/-小鼠随机分成2组,高脂高胆固醇饮食组(H组)4只,给予含21%脂肪、1.25%胆固醇的饲料喂养14周,中等脂肪饮食组(M组)4只,给予含10%脂肪的饲料喂养14周,观察比较不同脂肪和胆固醇含量饲料喂养后的血生化、Lee's指数、体脂比、肝体比和动脉病理切片的变化。结果 M组和H组的血清生化、Lee's指数、体脂比、肝体比无明显差异,常规HE染色切片、油红O冰冻切片显示20周的M组和H组主动脉都处于动脉粥样硬化脂质条纹期,斑块中表达大量的巨噬细胞源性泡沫细胞,存在大量坏死核和胆固醇结晶体,H组的主动脉窦上形成动脉瘤,M组腹主动脉形成动脉瘤,H组主动脉窦斑块中的胆固醇结晶体含量多于M组。结论 2组不同脂肪和胆固醇含量饲料对于早期ApoE-/-小鼠动脉粥样硬化进程存在一定影响,但总体上变化不大。OBJECTIVE To explore the effect of different fat and cholesterol feedstuff on the ApoE-/- mice atherosclerosis. METHODS Eight 6-week old mice were randomly put into two even groups. The High fat and cholesterol group （H ＇group） were given 21 % fat, 1.25 % cholesterol feedstuff for 14 weeks, and the Middle fat and cholesterol group （M group） were given 10 % fat for 14 weeks. Then observe the changes of blood biochemistry, Lee＇s index, and body fat percentage and Artery pathological section. RESULTS The results shows H group and M group have not much difference on the blood biochemistry, Lee ＇s index, body fat percentage and Artery pathological section. Both the routine HE dyeing section, oil red O staining show the H group and M group were in atheroselerosis fatty streak period, and a lot of macrophage macrophage foam cells were expressed in the plague, a large amount of necrotic corn and cholesterol crystals were existed. H group had arterial aneurysm in the aortic sinus, and M group had arterial aneurysm in the aorta abdominalis, and the cholesterol crystals in the H group are much more than the M group. CONCLUSION Both the two groups of different fat and cholesterol feedstuff have effect on the ApoE-/- mice atherosclerosis, but the influence is not that much.

关 键 词：动脉粥样硬化 动脉瘤 主动脉窦 饲料 

分 类 号：R965.1[医药卫生—药理学]