黄连素对人肝癌HepG2细胞增殖及血管内皮生长因子表达的影响  

Effects of berberine on the expression of vascular endothelial growth factor in HepG2 cells

作　　者：汪玉芳[1] 柯善栋[1] 陶秀良[2] 

机构地区：[1]黄石市中心医院血液科,湖北黄石435000 [2]华中科技大学同济医学院附属同济医院中西医结合科,湖北武汉430030

出　　处：《中国中西医结合消化杂志》2013年第3期143-145,共3页Chinese Journal of Integrated Traditional and Western Medicine on Digestion

摘　　要：[目的]研究黄连素对人肝癌HepG2细胞增殖和血管内皮细胞生长因子(vascular endothelial growth fac-tor,VEGF)表达的影响。[方法]分别以不同浓度(10、25、50、100μmol/L)的黄连素作用HepG2细胞24h后,采用MTT法观察细胞增殖变化、实时定量PCR(Real-time PCR)检测VEGF mRNA表达水平变化、Western blot检测VEGF蛋白水平变化。[结果]黄连素可抑制HepG2细胞的增殖,并呈浓度依赖性;不同浓度的黄连素作用细胞24h后,HepG2细胞VEGF mRNA和蛋白表达均有所下降,且呈现出一定的量效关系。[结论]黄连素可抑制人肝癌HepG2细胞的增殖和VEGF的表达,抗血管生成可能是黄连素抗肝肿瘤的作用机制之一。[Objective]To study the effect of berberine on cell proliferation and the expression of vascu- lar endothelial growth factor（VEGF） in HepG2 cells. [Methods]After being treated with different concen- trations of berberine for 24h, HepG2 cells growth inhibition was assessed by MTT assay. VEGF levels of mRNA and protein in HepG2 were detected by Real-time PCR and Western blot respectively. [Results]In certain concentration range, berberine inhibited cellular proliferation and down-regulated the mRNA and protein expression of VEGF in dose-dependant manner. [Conclusion]Berberine can inhibit the proliferation of HepG2 cells and expression of VgGF,and anti-angiogenesis may be one of the mechanisms of its antitumor action.

关 键 词：黄连素 肝细胞癌 血管内皮生长因子 

分 类 号：R282.71[医药卫生—中药学] R735.7[医药卫生—中医学]