不同处理方法对穿龙薯蓣种子萌发的影响  

Effects of different treatments on seed germination of Dioscorea nipponica

作　　者：杨利民[1] 宋波[1] 韩梅[1] 林春新[1] 

机构地区：[1]吉林农业大学中药材学院,吉林长春130118

出　　处：《中草药》2013年第6期755-759,共5页Chinese Traditional and Herbal Drugs

基　　金：国家科技重大专项(2011ZX09401-305-10-03);吉林省科技发展计划重大项目(20075022)

摘　　要：目的研究低温砂层积处理后不同发芽温度(10、15、20、25、30℃)及不同质量浓度的赤霉素(GA3)和萘乙酸(NAA)对穿龙薯蓣种子萌发的影响。方法采用培养皿培养法,对种子设置低温层积、不同质量浓度的GA3和NAA浸种处理,观察记录种子发芽率和发芽势并进行统计分析。结果穿龙薯蓣种子经4℃低温砂层积后,在25℃条件下发芽率和发芽势最高,分别为80.00%和53.33%;而采用100 mg/L GA3直接浸泡未层积种子24 h,发芽率和发芽势可达66.65%和48.35%;1 mg/L NAA浸泡12 h发芽率和发芽势为58.35%和43.35%;采用低温砂层积发芽进程最快,种子第6天开始发芽,第12天发芽完毕。结论采用4℃低温砂层积60 d后在25℃下进行催芽或采用100 mg/L GA3进行催芽处理有利于穿龙薯蓣种子萌发。Objeetive To study the effects of different temperature conditions （1 0, 15, 20, 25, and 30℃） and different concentration of gibberellin （GA3） and naphthalene acetic acid （NAA） on the germination responses ofDioscorea nipponica seeds. Methods The seed germination rate and germination vigor were recorded and analyzed statistically, through setting conditions including cold stratification and soaking treatment with different concentration of GA3/NAA in culture dishes. Results After sand stratification at 4 ~C, the germination percentage and germination vigor of D. nipponica seeds were the highest of 80.00% and 53.33% at 25 ℃, respectively. After soaking in 100 mg/L GA3 for 24 h without stratification, the germination percentage and vigor ofD. nipponica seeds were 66.65% and 48.35%, respectively; After soaking in 1 mg/L NAA for 12 h, the germination percentage and germination vigor were 58.35% and 3.35%, respectively. The germination process treated with low temperature sand stratification was the fastest. The seeds began to germinate at the day 6, and germinated completely within 12 d. Conclusion The seeds should germinate at 25 ℃ after sand stratification at 4 ℃ for 60 d, or the seeds without stratification would germinate after being treated with 100 mg/L GA3.

关 键 词：穿龙薯蓣 层积 赤霉素 萘乙酸 种子萌发 

分 类 号：R282.2[医药卫生—中药学]