PARP-1抑制剂5-AIQ联合依托泊苷对PC3细胞增殖的影响  

Effect of PARP-1 inhibitor 5-AIQ combined with Etoposide on the proliferation of prostate cancer cell line PC3

作　　者：朱寒亮[1] 吴文起[1] 段小鹿[1] 孔珍珍[1] 李舒珏[1] 梁叶萍[1] 

机构地区：[1]广州医学院第一附属医院微创外科中心泌尿外科广东省泌尿外科重点实验室,广东广州510230

出　　处：《海南医学》2013年第7期937-939,共3页Hainan Medical Journal

基　　金：国家自然科学基金青年基金(编号:30901495);广东省自然科学基金(编号:S2012010008674);广州市科技和信息化局计划项目(编号:2011J4100049)

摘　　要：目的研究多聚腺苷二磷酸核糖聚合酶-1(PARP-1)抑制剂5-氨基异喹啉酮(5-AIQ)联合化疗药物依托泊苷(VP16)对雄激素非依赖性前列腺癌PC3细胞株增殖的影响。方法 MTS比色法观察不同浓度5-AIQ联合VP16对PC3细胞的增殖抑制作用,并应用WesternBlot法检测应用5-AIQ或VP16及二者联合应用对PC3细胞内PARP-1蛋白表达的影响。结果 MTS结果显示,与单独应用VP16(5μmol/L、25μmol/L或125μmol/L)比较,联合应用5-AIQ(125μmol/L或500μmol/L)可以明显增强VP16对PC3细胞的增殖抑制作用,组间差异均有统计学意义(P<0.05)。而且当5-AIQ与低剂量VP16(5μmol/L)联合应用时可以达到与高剂量VP16(25μmol/L或125μmol/L)类似甚至更强的增殖抑制作用。WesternBlot结果显示,与对照组比较,5-AIQ(250μmol/L)或VP16(50μmol/L或100μmol/L)均可以明显下调PC3细胞内源性PARP-1的表达(P<0.05)。与二者单一处理组(5-AIQ,250μmol/L)或(VP16,50μmol/L)相比,二者联合应用可进一步下调PARP-1的表达水平。结论 PARP-1抑制剂5-AIQ可以明显增强VP16对PC3细胞的增殖抑制作用,该作用可能与PARP-1蛋白的表达抑制有关。Objective To observe the effect of PARP-1 inhibitor 5-AIQ combined with Etoposide VP16 on the proliferation of prostate cancer cell line PC3. Methods MTS assay was employed to analyze the effect of 5-AIQ and/or VP16 on the proliferation of PC3 cell. The expression level of PARP-1 was detected by Western Blot. Results MTS assay showed the combination of 5-AIQ and VP16 could significantly enhance the cytostatic action than VP16 alone （P〈0.05）. And the role of cytostatic action when treated with 5-AIQ combined with low-dose VP16 （5 μmol/L） was similar or stronger compared with high-dose VP16 （25 μmol/L or 125 μmol/L）. The expression levels of PARP-1 were significantly down-regulated when treated with 5-AIQ or VP16 alone （P〈0.05）. A further decrease of PARP-1 was found when treated with the combination of 5-AIQ and VP16. Conclusion 5-AIQ could significantly enhance the cytostatic action of VP16 to PC3 cells, which maybe related to the inhibition of PARP-1 expression.

关 键 词：前列腺癌 多聚腺苷二磷酸核糖聚合酶-1 5-氨基异喹啉酮 依托泊苷 增殖 

分 类 号：R329.25[医药卫生—人体解剖和组织胚胎学]