RP-HPLC法测定化生平合剂中黄芪甲苷的含量  

Content Determination of AstragalosideⅣ in Hua Sheng Ping Composition by RP-HPLC

作　　者：王娜[1] 席枝侠[1] 车晓侠[1] 仵文英[1] 徐晓娜[2] 冯伟[1] 

机构地区：[1]西安交通大学医学院第二附属医院药剂科,陕西西安710004 [2]西安交通大学医学院药学系,陕西西安710049

出　　处：《现代中药研究与实践》2013年第1期62-65,共4页Research and Practice on Chinese Medicines

摘　　要：目的建立化生平合剂中黄芪甲苷的含量测定方法。方法采用高效液相色谱法,KromasilC18色谱柱(4.6 mm×250 mm,5μm),流动相为乙腈-水-磷酸(1:2:0.15),检测波长为203nm,流速为1.0mL.min-1,柱温为室温。结果黄芪甲苷在进样量0.5-10μg范围内线性关系良好,r=0.999 6;平均回收率为97.82%,RSD为1.79%。结论测定结果准确、可靠,有良好的精密度和重现性,可用于化生平合剂中黄芪甲苷的含量测定。Objective To establish the method for determination of astragalosidelV in Hua Sheng Ping composition. Method The contents of astragalosidelV in Hua Sheng Ping composition were determined by HPLC. Kromasil C 18 column （4.6 mm250 mm, 5 μm） was used with acetonitrile-water-phosphoric acid （1：2：0.15） as mobile phase, and the detective wavelength was 203nm. The flow rate was 1.0 mL-min-1 and the column temperature was room temperature. Results AstragalosidelV had a good linear relation in the range of 0.5-10μg （r=0.999 6） and the average recovery was 97.82%, RSD was 1.79%. Conclusion The method has good precision and reproducibility, and the results were reliable and accurate. It could be used to determine the contents of astragalosidetV in Hua Sheng Ping composition.

关 键 词：化生平合剂 高效液相色谱法 黄芪甲苷 

分 类 号：R927.2[医药卫生—药学]