两种不同mGluR5抑制剂对创伤后Homer1a蛋白表达水平影响的研究  

Effect of Two Different mGluR5 Inhibitors on Post-traumatic Homer1a Protein Expression Level

作　　者：霍恺[1] 费舟[1] 刘藻斌[1] 陈涛[1] 罗鹏[1] 张磊[1] 赵明明[1] 朱杰[1] 刘文博[1] 蒋晓帆[1] 姬西团[1] 

机构地区：[1]第四军医大学西京医院神经外科,陕西西安710032

出　　处：《现代生物医学进展》2013年第5期801-803,903,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(30930093)

摘　　要：目的:研究代谢型谷氨酸受体5(mGluR5)的激动剂依赖性活性和激动剂非依赖性对创伤性脑损伤后Homer1a蛋白表达水平的影响。方法:离体培养神经元2 w,采用mGluR5抑制剂2-甲基-6-苯基乙炔基嘧啶(MPEP)和α-甲基-4-羧苯基甘氨酸(MCPG)两种不同剂量预处理神经元细胞1 h,神经元机械性损伤模型划伤细胞;利用立体定向仪固定大鼠,在大鼠脑皮层注射两种不同剂量的抑制剂,1 h后采用自由落体损伤模型造成大鼠颅脑损伤。通过Western blot分别检测离体和在体Homer1a蛋白表达水平的变化。结果:两种不同剂量MPEP预处理后,大鼠脑皮层和离体培养的神经元细胞中Homer1a蛋白表达水平表达明显减少,具有统计学差异(P<0.05);而两种不同剂量的MCPG预处理后,Homer1a蛋白表达水平的改变不明显。结论:颅脑损伤后内源性Homer1a的产生是与代谢型谷氨酸受体激动剂非依赖性活性密切相关。Objective： To investigate the effect of metabotropic glutamate receptor 5（mGluR5） agonist-dependent activity and agonist-independent activity on expression level of Homer1a protein after traumatic brain injury.Methods： Cortical neurons were cultured for 2 weeks,with two different doses of pretreatment of neurons,the mGluR5 inhibitor,2-methyl-6-phenyl ethynyl pyrimidine（MPEP） and α-methyl-4-carboxyphenyl glycine（MCPG） for one hour,and neurons mechanical injury model scratched cells;to fix the rat by a stereotactic frame,then injected the rat with two different doses of inhibitors.One hour later,the rat＇ s brain was injured because of free-fall injury model.Detected the changes of protein expression both.in vitro and vivo Homer1a by Western blot.Results： Homer1a expression was significantly reduced after pretreatment,by two different doses of MPEP,and there was significant difference（P 0.05）;But the changes of Homer1a after the MCPG pretreatment was not obvious.Conclusions： The expression of Homer1a after traumatic brain injury is closely related with agonist-independent activation of the metabotropic glutamate receptor 5.

关 键 词：代谢型谷氨酸受体5 抑制剂 Homerla 活性 

分 类 号：Q95-3[生物学—动物学] R651.15[医药卫生—外科学]