早期自然流产绒毛组织miR-10b表达及意义  

Expressions of miR-10b in Human Chorionic Villus During Early Spontaneous Abortion

作　　者：李怡[1] 吴涛[2] 栾丽霞[1] 张蓓蕾[1] 李三阳[1] 尹国武[1] 

机构地区：[1]第四军医大学唐都医院妇产科,陕西西安710038 [2]第四军医大学唐都医院普通外科,陕西西安710038

出　　处：《现代生物医学进展》2013年第4期620-623,650,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金青年基金项目(81170582);陕西省科技攻关项目(2011K15-02-04)

摘　　要：目的:探讨miR-10b在早期自然流产绒毛中的表达及其临床意义。方法:应用实时荧光定量PCR(real-time PCR)检测早期自然流产患者绒毛及正常早孕绒毛中miR-10b的表达水平;在滋养细胞系HTR8中上调miR-10b,研究miR-10b过表达滋养细胞的浸润功能改变。结果:①real-time PCR检测显示,miR-10b在早期自然流产患者绒毛表达(2.916±0.061)与正常早孕绒毛对照组(1.0±0.106)相比增高,差异有统计学意义(P<0.001)。②过表达miR-10b后,侵袭实验证实滋养细胞侵袭力明显增强。结论:miR-10b可能促进了滋养细胞浸润功能,参与了早期自然流产的病理过程。Objective： To study the expressions of human microRNA-10b（miR-10b） in human chorionic villus during early spon-taneous abortion.Methods： Real-time PCR method was used to study the distribution and changes of the miR-10b in the cells of human chorionic villus from the 5th to the 9th week of pregnancy and early spontaneous abortion.The synthesized miR-10b was transfected into HTR8 cells.The invation of miR-10b cells were investigated by Transwell assay.Results： The expression of miR-10b in ESA group in-creased significantly compared with that in normal pregnancy group（P 0.001）.Higher expression of miR-10b promoted the invation of HTR8 cells respectively.Conclusion： miR-10b play an important role during early unknown spontaneous abortion.

关 键 词：微小RNA MIR-10B 自然流产 滋养细胞 

分 类 号：R714.21[医药卫生—妇产科学]