N-乙酰半胱氨酸对脂多糖诱导的急性肺损伤大鼠肺组织病理形态及TGF-β1表达的作用  

Effects of N-Acetylcysteine on Pathomorphology and Expression of TGF-β1 in Lipopolysaccharide-Induced Acute Lung Injury in Rats

作　　者：蒋永亮 戴爱国 陈云荣 曾丹 李柏完[2] 

机构地区：[1]湖南省老年医院-湖南省老年医学研究所呼吸疾病研究室,湖南长沙410001 [2]南华大学附属第三医院呼吸内科,湖南衡阳421900

出　　处：《现代生物医学进展》2013年第4期654-657,共4页Progress in Modern Biomedicine

基　　金：湖南省医药卫生科研基金项目(B2008-065)

摘　　要：目的:观察N-乙酰半胱氨酸(NAC)对脂多糖诱导的急性肺损伤大鼠肺组织形态及转化生长因子-β1表达的作用。方法:将30只SD大鼠随机分为正常对照组、模型组和NAC干预组3组,每组10只。模型组经舌下静脉注射5 mg/kg脂多糖制备大鼠急性肺损伤模型,NAC干预组在注射脂多糖后lh腹腔注射NAC(200 mg/kg),注入脂多糖后12 h处死大鼠,取左叶肺组织,观察各组大鼠肺组织病理形态和TGF-β1的表达变化。结果:模型组肺泡间隔增宽,腔内有少许出血,渗出及炎性细胞浸润,肺间质充血水肿,有大量炎性细胞浸润,NAC干预组肺部炎性细胞浸润、渗出、出血较模型组明显减轻。模型组肺组织TGF-β1的表达较正常对照组明显增加(P<0.05),NAC干预组TGF-β1的表达较模型组显著降低(P<0.05),但与正常对照组比较差异无统计学意义。结论:NAC可显著减轻脂多糖诱导的大鼠急性肺损伤,这可能与其降低肺组织中TGF-β1的表达有关。Objective： To observe the effects of n-acetylcysteine on pathomorphology and expression of TGF-β1 in lipopolysac-charide（LPS）-induced acute lung injury（ALI） in rats.Methods： Thirty male SD rats were randomly divided into three groups： normal con-trol group,model group and NAC intervention group,ten rats in each group.The model of ALI was established by sublingual vein injec-tion of LPS（5 mg/kg）,NAC（200 mg/kg） was given 1h after injection of LPS,all the rats were sacreficed 12h after injection of LPS.The pathomorphology and expression of TGF-β1 in lung were observed and compared.Results： It was found in medel group that pulmonary alveolus interval had been broadened,and had many lymphocytes and monocytes infiltrated,pulmonary interstitial had congestion and edema,as well as large amount of inflammatory cell infiltration.While in NAC intervention group,inflammatory cell infiltration,exuda-tion,bleeding relieved.The expression of TGF-β1 in lung of model group was significantly higher than control group（P0.05）,the ex-pression of TGF-β1 in NAC intervention group was significantly lower than that in model group（P0.05）,but no significant difference was observed in comparison with control group.Conclusion： NAC could significantly relieve LPS-induced ALI in rats,the decrese of ex-pression of TGF-β1 in lung may be involved.

关 键 词：N-乙酰半胱氨酸 急性肺损伤 转化生长因子-β1 脂多糖 

分 类 号：Q95-3[生物学—动物学] R563.8[医药卫生—呼吸系统]