狼疮性肾炎患者淋巴细胞白细胞介素-6受体(CD_(126)与CD_(w130))分布的研究  

Distribution of interleukin-6 receptors (CD_(126) and CD_(w130) ) on circulating lymphocytes in patients with lupus nephritis

作　　者：曾丽霞[1] 孙林[1] 叶任高[1] 许韩师[1] 余学清[1] 孔庆瑜[1] 周飞宇[1] 

机构地区：[1]中山医科大学第一附属医院肾内科,广州510080

出　　处：《中华内科杂志》2000年第9期603-606,I020,共4页Chinese Journal of Internal Medicine

摘　　要：目的　了解狼疮性肾炎 (LN)患者外周血淋巴细胞白细胞介素 6 (IL 6 )受体 (CD12 6与CDw130 )的分布情况及其意义。方法　采用流式细胞术结合微量全血双重荧光标记法检测LN患者CD+4 、CD+8与CD+2 3 淋巴细胞表面CD12 6与CDw130 蛋白表达情况。结果　 (1)与正常对照组 (11例 )比较 ,活动组 (14例 )与非活动组 (10例 )LN患者CD+4 细胞百分数明显降低 (均P <0 0 1) ,CD+8细胞百分数显著增高 (P <0 0 1;P <0 0 5 ) ,CD+2 3 细胞百分数在活动组明显升高 (P <0 0 5 ) ,在非活动组明显降低(P <0 0 1) ,三组CD+12 6细胞与CD+w130 细胞百分数差异均无显著性 (P >0 0 5 )。 (2 )与非活动组和正常组比较 ,活动组LN患者CD+4 CD+12 6与CD+4 CD+w130 的细胞百分数明显降低 (P <0 0 5 ;P <0 0 1) ,CD+2 3CD+12 6与CD+2 3CD+w130 的细胞百分数则明显增高 (均P <0 0 5 ) ;非活动组和正常组比较差异则无显著性(P >0 0 5 ) ;CD+8CD+12 6与CD+8CD+w130 的细胞百分数 ,三组比较差异均无显著性 (P >0 0 5 )。 (3)活动组LN患者CD+2 3 细胞表面CD12 6与CDw130 表达呈正相关 (r =0 75 6 ,P <0 0 5 ) ;非活动组和正常组CD+2 3 细胞表面CD12 6与CDw130 表达无显著相关性 (r=0 376 ,P >0 0 5 ;r=0 2 5 6 ,P >0 0 5 )Objective To study the distribution of IL 6 receptors (CD 126 and CD w130 ) on lymphocytes in patients with lupus nephritis(LN). Method The percentages of lymphocyte subsets were examined by direct two colour immunofluorescence on flow cytometry. Results (1) In active and inactive cases, the percentages of CD + 4 cells obviously decreased as compared with the normal controls ( P <0 01). Increased numbers of CD + 8 cells could be detected in LN patients ( P <0 05), especially in active cases ( P <0 01). Lymphocytes of active cases contained significantly greater percentage of CD + 23 cells than those of normal subjects ( P <0 05). On the contrary,the percentage of CD + 23 cells was statistically lower in the inactive cases than in normal subjects ( P <0 01). No significant difference in the percentages of CD + 126 and CD + w130 cells was observed among the active , inactive and healthy groups ( P >0 05); (2) The proportion of CD + 4CD + 126 and CD + 4CD + w130 cells in total lymphocytes was significantly lower in the active than in the inactive ones or normal subjects ( P <0 05; P <0 01), whereas that of CD + 8CD + 126 and CD + 8CD + w130 cells was not different among the active, inactive and normal subjects ( P >0 05). The proportion of CD + 23 CD + 126 cells and CD + 23 CD + w130 cells augmented significantly in the active ( P <0 05), whereas it lessened in the inactive without statistical meaning as compared with the normal subjects ( P >0 05). (3) There was a postive correlation between the percentage of CD + 23 CD + 126 cells and that of CD + 23 CD + w130 cells in total lymphocytes in the active group ( r =0 756, P <0 05). However, no correlation between the percentage of CD + 23 CD + 126 cells and that of CD + 23 CD + w130 cells in total lymphocytes was found in the inactive ( r =0.376, P >0.05) or normal group ( r =0 256,

关 键 词：狼疮性肾炎 淋巴细胞 流式细胞术 IL-6R 

分 类 号：R593.2[医药卫生—内科学]