牡蛎壳粉/α-半水硫酸钙复合人工骨修复兔股骨髁缺损的实验研究  

Preparation of Oyster Shell Powder/α-CSH Combined Bone Graft and Its Related Research

作　　者：寇冬权[1] 林忠勤[2] 王伟[2] 程少文[2] 陈庆玉[2] 张伟[2] 彭磊[2,3] 

机构地区：[1]重钢总医院康复科,408000 [2]温州医学院附属第二医院,325027 [3]海南医学院附属医院创伤中心,570100

出　　处：《医学研究杂志》2013年第3期73-76,共4页Journal of Medical Research

基　　金：浙江省自然科学基金资助项目(Y405476)

摘　　要：目的评价牡蛎壳粉/α-半水硫酸钙复合人工骨材料填充兔股骨髁缺损的成骨能力及降解性。方法用牡蛎壳粉及α-半水硫酸钙按1∶3比例混合,制备人工骨。采用新西兰大白兔制做股骨髁缺损的动物模型,将复合人工骨植于一侧股骨髁,另一侧为空白对照,分别于术后4、8、12周取材、脱钙、组织包埋、切片行HE染色,观察成骨及材料降解情况。结果大体观察未见组织炎症反应,缺损区内填充的复合人工骨逐渐降解。4周时实验组有少量新骨形成,对照组无明显成骨,到8周时实验材料几乎降解完全,有部分骨小梁形成,对照组有少量新骨形成,生长部位主要在髓腔附近。在12周时实验组骨组织已经较为生熟,对照组有贴壁生长的骨小梁组织。经骨量评估发现在不同时间点,实验组骨量均高于对照组。结论 牡蛎壳粉/α-半水硫酸钙复合人工骨材料成骨能力及降解能力良好。Objective To evaluate the osteoconduction and degradation of Oyster shell powder/α - CSH combined bone graft in fe- mur defect of rabbits. Methods The oyster shell and ct - CSH were mixed into absolute ethyl alcohol,with mass ratio of 1：3, then injec- ted into mould. NewZealand rabbits were used to produce the defect of femur . The combined bone graft was randomly implanted one fe- mur, and the other femur was implanted nothing. After 4,8,12 weeks, the HE was used to obsever the osteogenesis and degradation of ma- terial. Results The implantation didn＇t lead to joint effusion and inflammation and the combined bone graft gradually degradation. With the degradation of materrial,the new bone gradually grew into the extension of the bone tissue, new bone mature trabecular bone at 12 weeks. And in control group new bone was form only at the edge of the defect, the density of central defects was almost near the denstity of hollow pith. At point - in - time, the result of forming new bone the experimental group was better than the control group, especially to the late. Conclusion Oyster shell powder/a - CSH combined bone graft is a biocompatible and biodegradable material and osteoconduc- tion is the main healing style in the bone defects.

关 键 词：牡蛎壳 α-半水硫酸钙骨缺损成骨降解 

分 类 号：R589.2[医药卫生—内分泌]