RhoA在损伤脊髓内表达的时空分布(英文)  

Temporal and spatial pattern of RhoA expression in injured spinal cord of adult mice

作　　者：康晓宁[1] 文锦坤[1] 王祥海[1] 潘梦婕[1] 张玮玮[1] 占小多[1] 刘忠英[1] 吴武田[2,3] 郭家松[1] 

机构地区：[1]南方医科大学基础医学院组织学与胚胎学教研室,广东广州510515 [2]香港大学李嘉诚医学院解剖学系,中国香港 [3]香港大学-暨南大学脑功能与健康联合实验室,广东广州510632

出　　处：《南方医科大学学报》2013年第4期463-468,共6页Journal of Southern Medical University

基　　金：Supported by National Natural Science Foundation of China(30973095)~~

摘　　要：目的研究脊髓损伤后不同时期RhoA在脊髓不同部位各种细胞中的表达。方法建立小鼠脊髓全横断性脊髓损伤模型,术后1,3,7,14,28,56,112 d后取材制备脊髓冰冻切片,利用RhoA/NF200、RhoA/GFAP、RhoA/CNPase和RhoA/IBA1双荧光标记免疫组织化学分别显示RhoA在神经元、星型胶质细胞、少突胶质细胞及小胶质细胞中的表达,并对各种细胞中RhoA阳性细胞百分率及RhoA阳性细胞的荧光强度进行定量分析。结果在正常小鼠脊髓内,只有极少数神经元或少突胶质细胞呈RhoA弱阳性表达。脊髓损伤后,神经元、星型胶质细胞及少突胶质细胞中的RhoA阳性率及荧光强度均显著增高并在损伤后第7天达到峰值,随后又逐渐降低,在损伤后112 d接近正常水平。小胶质细胞中RhoA的表达方式略有不同,RhoA阳性率及荧光强度均在第14天达到峰值,随后虽降低但在第112天仍保持较高的水平。结论创伤不仅导致脊髓神经元中RhoA阳性细胞数的明显增加和RhoA表达强度的提高,还可不同程度地增强各种胶质细胞中的RhoA表达。在不同时间段,不同部位及不同细胞内RhoA表达的方式均有不同。Objective To quantitatively analyze the temporal and spatial pattern of RhoA expression in injured spinal cord ofadult mice.Methods A spinal cord transection model was established in adult mice.At 1,3,7,14,28,56 and 112 days after thesurgery,the spinal cords were dissected and cryosectioned for RhoA/NF200,RhoA/GFAP,RhoA/CNPase or RhoA/IBA1 doublefluorescent immunohistochemistry to visualize RhoA expressions in the neurons,astrocytes,oligodendrocytes and microglia.The percentages as well as the immunostaining intensities of RhoA-positive cells in the parenchymal cells were quantitativelyanalyzed.Results RhoA was weakly expressed in a few neurons and oligidendrocytes in normal spinal cord.After spinal cordinjury,the percentage of RhoA-positive cells and RhoA expression intensity in the spinal cord increased and peaked at 7 dayspost injury（dpi） in neurons,oligodendrocytes and astrocytes,followed by a gradual decrease till reaching a low level at 112dpi.In the microglia,both the RhoA-positive cells and RhoA expression intensity reached the maximum at 14 dpi andmaintained a high level till 112 dpi.Conclusion Traumatic spinal cord injury can upregulate RhoA expression in the neuronsas well as all the glial cells in the spinal cord.RhoA expression patterns vary with post-injury time,location and amongdifferent parenchymal cells in the injured spinal cord.

关 键 词：脊髓损伤 神经元 星形胶质细胞 少突胶质细胞 小胶质细胞 RHOA 时空分布 

分 类 号：R651.2[医药卫生—外科学]