胰岛素联合生长激素上调脓毒症大鼠肝生长激素受体的表达  

The combination of insulin and growth hormone upregulates growth hormone receptor in septic rats

作　　者：殷红珍[1] 虞文魁[1] 陈启仪[1] 李宁[1] 朱维铭[1] 李维勤[1] 高涛[1] 张娟娟[1] 习丰产[1] 

机构地区：[1]南京大学医学院临床学院(南京军区南京总医院)解放军普通外科研究所,江苏南京210002

出　　处：《肠外与肠内营养》2013年第2期99-102,共4页Parenteral & Enteral Nutrition

基　　金：国家自然科学基金资助项目(81270854)

摘　　要：目的:观察脓毒症大鼠在胰岛素联合生长激素治疗前后生长激素受体的变化。方法:将56只造模成功大鼠随机分为七组,即对照组、脓毒症组、胰岛素组、生长激素组、联合组、LY294002组和MG-132组。于给药24 h后留取肝组织标本,检测生长激素受体mRNA和蛋白含量。结果:联合组较生长激素组脓毒症大鼠肝生长激素受体mRNA和蛋白含量增加。阻断PI3K和泛素蛋白酶体途径后,生长激素受体mRNA和生长激素受体蛋白均较联合组表达减少。结论:脓毒症时,胰岛素可通过PI3K信号上调肝细胞的生长激素受体水平,其作用可能与泛素蛋白酶体有关。Objective： To observe the effect of insulin combined with growth hormone to growth hormone receptor in the septic rats. Methods ： An hour after intraperitoneal injection of lipopolysaccharide （ 1 mg/kg） ,the survival SD rats would be conclued into our experiment. 56 septic rats were randomly divided into seven groups. Liver was collected after the experiment. Liver growth hormone receptor mRNA and protein content were detected. Results： When compared with the growth hormone group, the growth hormone receptor mRNA and protein content in the liver in group of insulin combined with growth hormone was significantly higher. After blocking PI3K, the expression and content in PI3K group were significantly lowerer. Conclusion： The insulin combined with growth hormone can increase the level of growth hormone receptor, and this may be related to the ubiquitin-proteasome. The PI3 K pathway may join this process.

关 键 词：脓毒症 生长激素受体 胰岛素 生长激素 

分 类 号：R459.7[医药卫生—急诊医学]