ω-3多不饱和脂肪酸对肝硬化大鼠肝切除术后肝细胞再生及肝纤维化程度的影响  

Omega-3 polyunsaturated fatty acids prevent progression of liver fibrosis and promote liver regeneration after partial hepatectomy in cirrhotic rats

作　　者：杨跃[1] 段飞[1] 蔡浩[1] 陈靓[1] 林建宇[1] 仇毓东[1] 

机构地区：[1]南京大学医学院附属鼓楼医院肝胆外科,江苏南京210008

出　　处：《肠外与肠内营养》2013年第2期103-106,共4页Parenteral & Enteral Nutrition

摘　　要：目的:观察ω-3多不饱和脂肪酸(ω-3PUFA)对肝硬化大鼠70%肝切除术后肝细胞再生及肝纤维化程度的影响。方法:将96只大鼠随机分为四组,即正常对照组(仅行70%肝切除);肝硬化对照组(肝硬化大鼠行70%肝切除);肝硬化小剂量组(肝硬化大鼠行70%肝切除后静脉注射ω-3PUFA 1 ml/kg);肝硬化大剂量组(肝硬化大鼠行70%肝切除后静脉注射ω-3PUFA 2 ml/kg)。观察大鼠术后第1、3、5和7天肝细胞再生的情况,肝功能指标,残肝肝纤维化程度的变化等。结果:与肝硬化对照组比,肝硬化大剂量组大鼠术后肝有明显的再生(P<0.05);术后第1和第3天ALT和AST有明显降低(P<0.05)。术后第7天,肝硬化小剂量组和肝硬化大剂量组肝纤维化程度有明显减轻(P<0.05)。结论:ω-3PUFA不仅能促进肝硬化大鼠术后肝细胞再生,保护术后肝功能恢复,而且能改善肝硬化大鼠术后肝纤维化程度。Objective ： To evaluate the effect of omega-3 polyunsaturated fatty acids （ ω-3 PUFA） on liver regeneration after hepatectomy and antifibrosis under the condition of liver cirrhosis in rats. Methods： Seventy precent hepatectomy was carried out in rats, which were subsequently divided into 4 groups： ①normal and hepatectomy group （PH）, ②liver cirrhosis and hepatectomy group（ LC ＋ PH）,③liver cirrhosis,n-3 PUFA （ 1 ml/kg） and hepatectomy group （ LC ＋ n-3 PUFA[ S] ＋ PH） ,④ liver cirrhosis, n-3 PUFA （2 mL/kg） and hepatectomy group （LC ＋ n-3PUFA[ L] ＋ PH）. Body/liver weight ratios, Serum parameters, histopathological examination, immunostaining and quantification of mRNA expression were also investigated. Results： Liver regeneration in LC ＋ PH group was significantly delayed compared with PH group 7 days after hepatectomy. On the other hand, liver regeneration in LC ＋ n-3 PUFA [ L ] ＋ PH group increased significantly. The liver fibrosis was significantly lower in the groups with use of n-3 PUFA. Conclusion ： The n-3 PUFA can reduce liver fibrosis and promote liver regeneration, even under cirrhotic conditions.

关 键 词：Ω-3多不饱和脂肪酸 70%肝切除 肝细胞再生 肝硬化 

分 类 号：R657.3[医药卫生—外科学]