叶酸受体靶向性的γ-环糊精-叶酸包合物修饰的CdSe/ZnS量子点的合成及其生物活性  

Synthesis and bioactivity of the folate receptor targeted γ-cyclodextrin-folate inclusion-coated CdSe/ZnS quantum dots

作　　者：赵美霞[1] 李洋[1] 王超杰[1] 

机构地区：[1]河南大学河南省天然药物与免疫工程重点实验室,河南开封475004

出　　处：《药学学报》2013年第4期566-572,共7页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金-河南人才培养联合基金(U1204201);中国博士后科学基金面上资助(2012M511570)

摘　　要：采用简单且方便的超声方法合成了具有叶酸受体(FR)靶向性的γ-环糊精-叶酸包合物(γ-CD/FA)修饰的CdSe/ZnS量子点(QDs)纳米粒。利用傅里叶红外光谱(FTIR)、核磁共振氢谱(1H NMR)、紫外可见吸收光谱(UV-vis)、荧光光谱和透射电子显微镜(TEM)等手段对其进行了表征。结果表明,FR靶向性的γ-CD/FA修饰的CdSe/ZnS QDs,不但具有较好的单分散性和较小的尺寸,还具有较高的量子产率(QY)和较长的荧光寿命等较好的光学性能。细胞毒性实验表明,FR靶向性的γ-CD/FA修饰的CdSe/ZnS QDs具有较低的毒性,还具有选择性地进入FR高表达的癌细胞内的功能。这种直径为4～5 nm的QDs比较容易进入细胞,在体内易被肾脏清除,比较适合于在生物探针或生物成像等生物医学方面的应用。The y-cyclodextrin-folate （y-CD/FA） inclusion-coated CdSe/ZnS quantum dots （QDs） with folate- receptor （FR） targeted were synthesized by simple and convenient sonochemical method. The products were studied using fourier transform infrared （FTIR）, proton nuclear magnetic resonance （ 1H NMR）, utraviolet-visible spectrometry （UV-vis）, fluorescence spectrum and transmission electron micrographs （TEM）. The results showed that the y-CD/FA-coated CdSe/ZnS QDs not only have good monodispersity and smaller size, but also have good optical performance, such as higher quantum yield （QY） and a long fluorescence lifetime. The cytotoxicity experiments showed that the y-CD/FA-coated CdSe/ZnS QDs have lower cytotoxicity and could more effectively enter cancer cells with FR over-expression. The QDs with 4-5 nm in diameter were relatively easy to enter the cell and to be removed through kidneys, so it is more suitable for biomedical applications for bioprobes and bioimaging.

关 键 词：量子点 叶酸 环糊精 肿瘤细胞 光学成像 

分 类 号：R943[医药卫生—药剂学]