内质网应激反应在大鼠Q开关1064nmNd：YAG激光非剥脱性嫩肤治疗机制中的作用  

Analysis of the endoplasmic reticulum stress in non-ablative skin rejuvenation using Q-switched 1064nm Nd：YAG laser

作　　者：李子全[1] 庄乐[3] 冯子超[1] 戚其超[1] 钟华[3] 马伟元[2,3] 

机构地区：[1]山东大学医学院,济南250012 [2]山东大学医学院病理学研究所 [3]山东大学齐鲁医院皮肤病科

出　　处：《中华整形外科杂志》2013年第2期113-116,共4页Chinese Journal of Plastic Surgery

基　　金：山东省科技发展计划（2012GSF11855）

摘　　要：目的探究Q开关1064nmNd：YAG激光照射对大鼠真皮胶原增生和皮肤中内质网分子伴侣（BiP／GRP78）表达水平的影响，探讨嫩肤治疗中内质网应激反应的作用机制。方法将25只Wistar大鼠背部皮肤脱毛，平均分成2部分，一部分用Q开关1064nmNd：YAG激光照射背部脱毛的大鼠皮肤共4次，每次间隔2d（实验组）；另一部分不做任何处理（未照射组）。取照射后第14和30天的大鼠皮肤组织进行HE染色，测量其真皮厚度；并用碱水解法检测羟脯氨酸含量。取照射后皮肤组织，采用免疫组织化学染色技术检测BiP／GRP78的表达。羟脯氨酸含量和真皮厚度使用t检验，免疫组化使用）（。检验，以P〈0．05为差异有统计学意义。结果激光照射第14和30天的大鼠皮肤真皮厚度明显增加，增加率分别为29．6％和16．7％，胶原纤维束增粗、密集，皮肤羟脯氨酸含量增加，与未照射组比较差异有统计学意义（P〈0．05）。免疫组化显示BiP／GRP78在激光照射后表达率为100％，较未照射组明显上调，差异有统计学意义（X2=28．76，P〈0．01）。结论Q开关1064nmNd：YAG激光照射大鼠皮肤，引发内质网应激反应，促进蛋白质正确折叠、合成，真皮胶原纤维增粗密集，从而达到非剥脱性嫩肤治疗的目的。Objective To study the effect of Q-switched 1064 nm Nd： YAG laser treatment on the proliferation of dermal collagen and expression of immunoglobulin binding protein/glucose related protein 78 （BiP/GRP78） in rats＇ skin and the mechanism of endoplasmic reticulum stress. Methods Dorsal skin of 25 Wistar rats was divided into two parts equally after hair removal. Q-switched 1064nm Nd： YAG laser was applied to treat rats＇ dorsal skin for 4 times at an interval of 2 days in the experiment part. The control part received no laser treatments. The rats＇ dorsal skin samples were taken on the 14th and 30th day after laser treatment to measure the dermis thickness and collagen bundles under HE stain and to measure the hydroxyproline content by alkaline hydrolysis method after laser treatment. The expression of BiP/GRP78 was also detected by immunohistochemical method. Statistics was used to analyze the data. Results The dermis thickness increased by 29.6% on the 14th day and 16.7% on the 30th day after laser treatment. The collagen bundles became thicker and denser. The hydroxyproline in the skin was also raised after laser treatment （P 〈 0.05）. The immunohistochemical result showed the expression of BiP/GRP78 increased to 100% after laser treatment, showing a significant difference from the control group（X2 = 28.76 ,P 〈 0.01 ）. Conclusions The Q-switched 1064 nm Nd：YAG laser treatment can induce endoplasmic reticulum stress, so as to enhance the protein folding and synthesizing precisely. The proliferation of dermis collagen is the base of effect of non-ablative skin rejuvenation.

关 键 词：内质网 分子监控蛋白类 钕 钇 铝石榴子石激光器 胶原 羟脯氨酸 

分 类 号：R622[医药卫生—整形外科]