DNA甲基转移酶1在人瘢痕疙瘩成纤维细胞中的表达及意义  

Expression and significance of DNMT1 in human keloid fibroblast

作　　者：杨娥[1] 邹崎葩[1] 张恒术[1] 

机构地区：[1]重庆医科大学附属第一医院整形美容科,重庆400016

出　　处：《中华整形外科杂志》2013年第2期117-120,共4页Chinese Journal of Plastic Surgery

摘　　要：目的探讨DNA甲基转移酶1（DNA methyltransferase1，DNMT1）在瘢痕疙瘩的不同生长期和不同部位的成纤维细胞表达及其意义。方法收集53例瘢痕疙瘩患者手术切除的瘢痕疙瘩标本，并按生长期分为A（〈6个月）、B（6～12个月）、C（1～2年）、D（〉2年）4组；免疫组织化学法检测各组标本正常皮肤、浸润部、增生部及老化部成纤维细胞DNMT1的表达并比较差异；RT-PCR法检测各组标本及不同部位成纤维细胞DNMT1mRNA的表达。结果DNMT1在成纤维细胞中主要分布于细胞核内；瘢痕疙瘩成纤维细胞DNMT1蛋白阳性表达率（72％）及DNMT1mRNA表达均高于正常皮肤成纤维细胞（8％，P=0．001）；瘢痕疙瘩生长期较短组成纤维细胞DNMT1阳性表达率（100％，评分：10．47±1．85）及DNMT1mRNA表达均高于生长期较长组（45％，评分：7．71±1．80，P=0．007）；瘢痕疙瘩浸润部（100％，评分：10．47±1．85）成纤维细胞DNMT1阳性表达率及DNMT1mRNA表达均高于增生部（78％，评分：6．63±1．75）与老化部（38％，评分：5．53±1．55，P=0．001）。结论DNMT1在瘢痕疙瘩发病中具有重要作用；随着瘢痕疙瘩生长期的延长，DNMT1的表达有降低趋势；随着瘢痕疙瘩中央成纤维细胞老化，DNMT1表达降低。提示DNMT1在瘢痕疙瘩生长过程中起着重要的作用，并且与瘢痕疙瘩浸润生长密切相关。Objective To investigate the expression and significance of DNA methyltransferase 1 （DNMT1） in different parts and formation time of keloid fibroblasts. Methods Specimen were collected from fifty-three patients with keloid. The keloids were grouped according to formation time. Immunohistochemistry was used to assay the expression of DNMT1 in normal skin, invasive part, proliferative part and aged part. RT-PCR was adopted to assay the expression of DNMT1 mRNA in each group. Results DNMT1 is mainly distributed in the nucleus of fibroblasts. The expression of DNMTI protein and DNMTlmRNA in keloid（72% ） is higher than that in normal skin fibroblasts（8% ） （P = O. 001）. The expression rate of DNMT1 protein and DNMT1 mRNA in shorter formation time group （ 100% ,Score： 10.47 ± 1.85 ） is higher than a longer formation time group（45% ,Score：7.71± 1.80） （ P =0. 007）. The expression rate of DNMT1 protein and DNMT1 mRNA in the invasive part of keloid （100% ,Score： 10. 47 ±1. 85） is higher than the proliferative part（78% , Score：＆ 63 ± 1.75） and aged part（38% ,Score ：5.53 ±1.55） （P = 0. 001 ）. Conclusions DNMTI plays an important role in keloid. The expression of DNMT1 tends to decrease along with the increase of the formation time in keloid. It is also related to the part of keloid. DNMT1 plays an important role in the process of keloid growth and is closely related to infiltrative growth in keloid.

关 键 词：瘢痕疙瘩 DNA甲基转移酶1 成纤维细胞 

分 类 号：R622[医药卫生—整形外科]