中药土鳖虫对兔下颌骨牵张成骨作用的研究  

Effect of Eupolyphaga Sinensis Walker on mandibular distraction osteogenesis in rabbits

作　　者：彭利伟[1] 杨海新[2] 

机构地区：[1]河南省人民医院口腔科,郑州450003 [2]郑州大学口腔医学院

出　　处：《中华整形外科杂志》2013年第2期125-130,共6页Chinese Journal of Plastic Surgery

基　　金：河南省自然科学基金资助项目（0611045500）;河南省卫生科技创新型人才工程专项经费资助（4022）

摘　　要：目的研究单味中药土鳖虫能否促进下颌骨牵张成骨过程中的骨形成。方法将30只日本大耳白兔（性别不限，体重2．0—2．5kg）随机分为实验组和对照组，每组各15只，均建立右侧兔下颌骨牵张成骨模型。实验组自牵张期第1天起每天喂食土鳖虫粉2g，直至处死；对照组正常饮食。于固定期24、72h，1、4和7周分别处死实验组和对照组大耳兔各3只，对标本进行大体观察、X线观察、组织学观察及骨形成蛋白（bone morphogenetic proteins，BMPs）、血管内皮生长因子（vascular endothelial growth factor，VEGF）免疫组织化学染色分析。结果实验组动物牵张间隙区内的新骨形成速度明显快于对照组，同一时期实验组成骨活动较对照组活跃；实验组BMPs及VEGF的表达明显高于对照组（P〈0．05）。结论中药土鳖虫可促进兔下颌骨牵张成骨过程中的骨形成，其作用机制可能与其促进BMPs及VEGF的表达有关。Objective To study the effect of Eupolyphaga Sinensis Walker on mandibular distraction osteogenesis （DO） in rabbits. Methods 30 Japanese white rabbits （weight 2. 0-2.5 kg, about 3 months old） were divided randomly into control group （n = 15） and experimental group （n = 15）. Unilateral mandibular DO models were established at the right mandible of the rabbits. Distraction was started 7 days after the surgery at the speed of 0. 4 mm per time twice a day and continued for 10 days.From the first day of distraction to the day of execution, the experimental group rabbits were fed with 2 g of ESW power once a day at 9 o＇ clock. Three animals in each group were executed respectively at 24 hours, 72 hours, 1 week, 4 weeks and 7 weeks after completion of distraction, and the specimens of DO were harvested. The general observation, X-ray examination, histological study and immunohistochemical staining of bone morphogenctic proteins （BMPs） and vascular endothelial growth factor （VEGF） were performed. The images of immunohistochemical staining of BMPs and VEGF were analyzed by the image analysis software, and the results were analyzed by statistical software SPSS 17.0. Results The rate of the new bone formation in the experimental group was faster than that in the control group, and the immunohistochemical staining of BMPs and VEGF in the experimental group was higher than that in the control group. Conclusions ESW can promote the formation of the new bone in the distracted gap during mandibular DO in rabbits, which may be due to its enhancement effect on the expression of BMPs and VEGF.

关 键 词：骨生成 牵张 土鳖虫 骨形成蛋白 血管内皮生长因子 

分 类 号：R285.5[医药卫生—中药学]